Directly conjugated antibodies for immunofluorescence

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Overview

  • Product namePAP pen

Properties

    Applications

  • Application notesThe PAP pen is a special marking pen that provides a thin film-like green-tinged hydrophobic barrier when a circle is drawn around a specimen on a slide.
    This water repellent barrier keeps staining reagents localized on the tissue sections, prevents mixing of reagents when differentially staining two sections on the same slide, and allows use of less reagents per section.
    Insoluble in alcohol and acetone, PAP Pen markings can be optionally removed with xylene after the staining procedure is complete.
    PAP Pen is good for approximately 500 uses.

    • PAP pen images

    • - PAP pen (ab2601)
      - PAP pen (ab2601)

    References for PAP pen (ab2601)

    This product has been referenced in:
    • Aydin K  et al. Effect of epidermal growth factor receptor status on the outcomes of patients with metastatic gastric cancer: A pilot study. Oncol Lett 7:255-259 (2014). Read more (PubMed: 24348859) »

    See 1 Publication for this product

    Publishing research using ab2601 ? Please let us know so that we can cite the reference in this datasheet

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    13 Abreviews or Q&A

    10
    Votes: Highest First

    Barrier pen

     Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    Precise and reliable barrier pen. After application it dries rather quick and after that there's no leakage. Found it very useful for IHC when I had to isolate from each other few tissue samples on the same slide. It resists incubation in buffers with Tween-20. Soluble in Xylene.
    Username

    Mr. Vasily Gnyubkin

    Verified customer

    Submitted May 02 2014

    Product code: 2601
    Inquiry: Hi could you please send me the MSDS for ab2601. Thanks

    Thank you for contacting us.

    The safety datasheet has published it should appear on the website shortly

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Product code: 2601

    Inquiry: Can I use your pap pen in immunohistochemistry protocols using triton X in wash buffer? will Triton X dissolve pen markings from the slide?



    The PAP pen will not get washed off by the wash buffer even if it has Triton in it.

    Can I use this PAP pen at temperatures around 200°C?

    Thank you for your enquiry.

    This ab2601 is also known as Super PAP Pen which outperforms the original PAP Pen. Itmay be used at temperatures as high as 48°C (120°F). The old style PAP Pen worked only up to 32°C (90°F). I am afraid that you w...

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    Can you please email me a copy of the MSDS for ab2601?

    Thank you for contacting us.

    Please find attached the MSDS for ab2601.

    Please do not hesitate to contact us if you need any more advice or information.

    Then I am back again.   Do you know the percentage of these two substances in the PAP pen?

    Unfortunately this is considered proprietary by our manufacturer and we are not able to provide this information. Apologies for any inconvenience.

    Could you provide me with the MSDS for ab2601

    Thank you for calling Abcam. Please find attached the MSDS for ab2601, PAP pen. If there is anything else I can do for you, please let me know.

    I'm after a mini pap pen, I think it draws a 2.5mm line rather than the usual 5mm. Is the Abcam pen one of these, or do you have others?

    Thank you for contacting us. The end of the Pap pen is about 3mm thick and tapers off to about 1mm. The PAP pen ab2601 is the only one we currently have in our catalog. I hope this information is helpful to you. Please do not hesitate to contact...

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    I have a customer who is interested in AB2601 PAP PEN, it is indicated that each unit is good for approximately 500 uses. The customer wants to know what is the volume in the per unit ? I am looking forward to your reply. .

    Thank you for your enquiry. We have not calculated the volume of liquid used per unit, however I can confirm that one unit is equivalent to drawing with the pen along the four sides of a standard IHC slide, such that a hydrophobic barrier is created...

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    Having problems finding the "old style" PAP pen, and I'm hoping that this is what you are selling. I need the pens that are labelled PAP PEN (white pen with a green label) and not the new kind that are actually called Liquid blocker. And since there is...

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    Thank you for your interest in our products. Regarding your enquiry, our PAP pen is white with a black label. The tip is green and the PAP line is light green. It is not known as a liquid blocker. I hope this information will be useful for you....

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    1-10 of 13 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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