Overview

  • Product name
    PAP pen
  • General notes
    The PAP pen is a special marking pen that provides a thin film-like green-tinged hydrophobic barrier when a circle is drawn around a specimen on a slide.

    This water repellent barrier keeps staining reagents localized on the tissue sections, prevents mixing of reagents when differentially staining two sections on the same slide, and allows use of less reagents per section.

    Insoluble in alcohol and acetone, PAP Pen markings can be optionally removed with xylene after the staining procedure is complete.

    PAP Pen is good for approximately 500 uses.

Properties

Images

  • Other - PAP pen (ab2601)
    Other - PAP pen (ab2601)
    PAP pen (ab2601)

References

This product has been referenced in:
  • Deng Y  et al. Improved i.p. drug delivery with bioadhesive nanoparticles. Proc Natl Acad Sci U S A 113:11453-11458 (2016). Read more (PubMed: 27663731) »
  • Aydin K  et al. Effect of epidermal growth factor receptor status on the outcomes of patients with metastatic gastric cancer: A pilot study. Oncol Lett 7:255-259 (2014). Read more (PubMed: 24348859) »

See all 2 Publications for this product

Publishing research using ab2601? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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15 reviews or Q&A

10
Votes: Highest First

super pen

 Excellent Excellent 5/5 (Ease of Use)
Abreviews
The pen works well. It`s easy to use and dries quickly. The barrier is precise and reliable - even after 24h incubation. It is resistant to buffer but can be removed with xylene.The pen is a great help in the IHC when there are multiple tissue samples on the same slide. And you save a lot of reagent, because you can frame the samples as small as possible.
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Abcam user community

Verified customer

Submitted Mar 06 2018

high quality PAP pen

 Excellent Excellent 5/5 (Ease of Use)
Abreviews
This PAP pen is very easy to use. It dries quickly. It is strong enough to create and maintain a boundary between tissue sections. I usually stain secondary antibody control on the same slide with primary antibody, so I divided the section with the PAP pen. After overnight incubation with antibody, the boundary still functional and there was no mixing between adjacent drop of solutions. Picture was taken after overnight incubation.
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Abcam user community

Verified customer

Submitted Dec 02 2016

PAP pen

 Good Good 4/5 (Ease of Use)
Abreviews
PAP pen dries quickly and forms a hydrophobic layer that is not washed off by 0.1% Triton X-100 in PBS. The only concern I have is that the barrier is often difficult to dry after washing, so extra care must be taken to avoid mixing reagents between adjacent sections.
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Mr. Bryan Niedenberger

Verified customer

Submitted Aug 31 2016

Could you please support with some information Hydrophobic Barrier Pen cat no AB2601.
what is the thickness of the pen.

I am happy to confirm that the tip of the Pap pen is about 3 mm thick and tapers off to about 1 mm.

Barrier pen

 Excellent Excellent 5/5 (Ease of Use)
Abreviews
Precise and reliable barrier pen. After application it dries rather quick and after that there's no leakage. Found it very useful for IHC when I had to isolate from each other few tissue samples on the same slide. It resists incubation in buffers with Tween-20. Soluble in Xylene.
Username

Mr. Vasily Gnyubkin

Verified customer

Submitted May 02 2014

Product code: 2601
Inquiry: Hi could you please send me the MSDS for ab2601. Thanks

Thank you for contacting us.

The safety datasheet has published it should appear on the website shortly

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Can I use this PAP pen at temperatures around 200°C?

Thank you for your enquiry.

This ab2601 is also known as Super PAP Pen which outperforms the original PAP Pen. Itmay be used at temperatures as high as 48°C (120°F). The old style PAP Pen worked only up to 32°C (90°F). I am afraid that you w...

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Can you please email me a copy of the MSDS for ab2601?

Thank you for contacting us.

Please find attached the MSDS for ab2601.

Please do not hesitate to contact us if you need any more advice or information.

Then I am back again.   Do you know the percentage of these two substances in the PAP pen?

Unfortunately this is considered proprietary by our manufacturer and we are not able to provide this information. Apologies for any inconvenience.

Could you provide me with the MSDS for ab2601

Thank you for calling Abcam. Please find attached the MSDS for ab2601, PAP pen. If there is anything else I can do for you, please let me know.

1-10 of 15 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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