Customer reviews and FAQs

Overview

  • Product namePAP pen
  • General notesThe PAP pen is a special marking pen that provides a thin film-like green-tinged hydrophobic barrier when a circle is drawn around a specimen on a slide.

    This water repellent barrier keeps staining reagents localized on the tissue sections, prevents mixing of reagents when differentially staining two sections on the same slide, and allows use of less reagents per section.

    Insoluble in alcohol and acetone, PAP Pen markings can be optionally removed with xylene after the staining procedure is complete.

    PAP Pen is good for approximately 500 uses.

Properties

    PAP pen images

    • Other - PAP pen (ab2601)
      Other - PAP pen (ab2601)
      PAP pen (ab2601)

    References for PAP pen (ab2601)

    This product has been referenced in:
    • Deng Y  et al. Improved i.p. drug delivery with bioadhesive nanoparticles. Proc Natl Acad Sci U S A 113:11453-11458 (2016). Read more (PubMed: 27663731) »
    • Aydin K  et al. Effect of epidermal growth factor receptor status on the outcomes of patients with metastatic gastric cancer: A pilot study. Oncol Lett 7:255-259 (2014). Read more (PubMed: 24348859) »

    See all 2 Publications for this product

    Publishing research using ab2601? Please let us know so that we can cite the reference in this datasheet.

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    14 Abreviews or Q&A

    10
    Votes: Highest First

    high quality PAP pen

     Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    This PAP pen is very easy to use. It dries quickly. It is strong enough to create and maintain a boundary between tissue sections. I usually stain secondary antibody control on the same slide with primary antibody, so I divided the section with the PAP pen. After overnight incubation with antibody, the boundary still functional and there was no mixing between adjacent drop of solutions. Picture was taken after overnight incubation.
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    Username

    Abcam user community

    Verified customer

    Submitted Dec 02 2016

    PAP pen

     Good Good 4/5 (Ease of Use)
    Abreviews
    PAP pen dries quickly and forms a hydrophobic layer that is not washed off by 0.1% Triton X-100 in PBS. The only concern I have is that the barrier is often difficult to dry after washing, so extra care must be taken to avoid mixing reagents between adjacent sections.
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    Username

    Mr. Bryan Niedenberger

    Verified customer

    Submitted Aug 31 2016

    Could you please support with some information Hydrophobic Barrier Pen cat no AB2601.
    what is the thickness of the pen.

    I am happy to confirm that the tip of the Pap pen is about 3 mm thick and tapers off to about 1 mm.

    Barrier pen

     Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    Precise and reliable barrier pen. After application it dries rather quick and after that there's no leakage. Found it very useful for IHC when I had to isolate from each other few tissue samples on the same slide. It resists incubation in buffers with Tween-20. Soluble in Xylene.
    Username

    Mr. Vasily Gnyubkin

    Verified customer

    Submitted May 02 2014

    Product code: 2601
    Inquiry: Hi could you please send me the MSDS for ab2601. Thanks

    Thank you for contacting us.

    The safety datasheet has published it should appear on the website shortly

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Can I use this PAP pen at temperatures around 200°C?

    Thank you for your enquiry.

    This ab2601 is also known as Super PAP Pen which outperforms the original PAP Pen. Itmay be used at temperatures as high as 48°C (120°F). The old style PAP Pen worked only up to 32°C (90°F). I am afraid that you w...

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    Can you please email me a copy of the MSDS for ab2601?

    Thank you for contacting us.

    Please find attached the MSDS for ab2601.

    Please do not hesitate to contact us if you need any more advice or information.

    Then I am back again.   Do you know the percentage of these two substances in the PAP pen?

    Unfortunately this is considered proprietary by our manufacturer and we are not able to provide this information. Apologies for any inconvenience.

    Could you provide me with the MSDS for ab2601

    Thank you for calling Abcam. Please find attached the MSDS for ab2601, PAP pen. If there is anything else I can do for you, please let me know.

    I'm after a mini pap pen, I think it draws a 2.5mm line rather than the usual 5mm. Is the Abcam pen one of these, or do you have others?

    Thank you for contacting us. The end of the Pap pen is about 3mm thick and tapers off to about 1mm. The PAP pen ab2601 is the only one we currently have in our catalog. I hope this information is helpful to you. Please do not hesitate to contact...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"