Overview

  • Product name
  • Description
    Rabbit polyclonal to PAPOLA
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Xenopus laevis
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 31-243 of Human PAPOLA (P51003).

  • Positive control
    • HepG2 whole cell lysate. IF/ICC: JEG3 cell line.
  • General notes
    Keep as concentrated solution.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78% PBS, 20% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab126934 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/3000. Predicted molecular weight: 83 kDa.

Target

  • Function
    Polymerase that creates the 3'-poly(A) tail of mRNA's. Also required for the endoribonucleolytic cleavage reaction at some polyadenylation sites. May acquire specificity through interaction with a cleavage and polyadenylation specificity factor (CPSF) at its C-terminus.
  • Sequence similarities
    Belongs to the poly(A) polymerase family.
  • Post-translational
    modifications
    Polysumoylated. Varying sumolyation depending on tissue- and cell-type. Highly sumoylated in bladder and NIH 3T3 cells. Sumoylation is required for nuclear localization and enhances PAP stability. Desumoylated by SENP1. Inhibits polymerase activity.
    Hyperphosphorylation on multiple CDK2 consensus and non-consensus sites in the C-terminal Ser/Thr-rich region represses PAP activity in late M-phase. Phosphorylation/dephosphorylation may regulate the interaction between PAP and CPSF.
    Acetylated in the C-terminus. Acetylation decreases interaction with NUDT21 and KPNB1, and inhibits nuclear localization through inhibiting binding to the importin alpha/beta complex.
  • Cellular localization
    Cytoplasm. Nucleus. The 90 kDa form is nuclear while the 100 kDa and the 106 kDa forms are both nuclear and cytoplasmic.
  • Information by UniProt
  • Database links
  • Alternative names
    • MGC5378 antibody
    • PAP alpha antibody
    • PAP antibody
    • PAP-alpha antibody
    • PapIII antibody
    • PAPOA_HUMAN antibody
    • PAPOLA antibody
    • Plap antibody
    • Poly(A) polymerase alpha antibody
    • Polynucleotide adenylyltransferase alpha antibody
    see all

Images

  • ab126934 stained JEG3 cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab126934 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Anti-PAPOLA antibody (ab126934) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size : 83 kDa
    7.5% SDS PAGE

References

This product has been referenced in:
  • Bresson SM & Conrad NK The human nuclear poly(a)-binding protein promotes RNA hyperadenylation and decay. PLoS Genet 9:e1003893 (2013). WB ; Human . Read more (PubMed: 24146636) »

See 1 Publication for this product

Customer reviews and Q&As


I am very pleased to hear you would like to accept our offer and test ab126934 in S pombe. This code will give you: 1 freeprimary antibodyOR $337.00 OFF OF ORDER before the expiration date. To redeem this offer, please submit an Abreview for [S po...

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We note the amino acids of the peptide immunogens used to produce each of those antibodies. Have you aligned the peptide sequences with the Poly A polymerase sequence in S. po...

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