• Product nameAnti-PAPOLA antibody
    See all PAPOLA primary antibodies
  • Description
    Rabbit polyclonal to PAPOLA
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide corresponding to a region between residue 694 and the C-terminus (residue 744) of human PAPOLA (NP_116021.2)

  • Positive control
    • HeLa whole cell lysate.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab72492 was affinity purified using an epitope specific to PAPOLA immobilized on solid support.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products


Our Abpromise guarantee covers the use of ab72492 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
  • Application notesIs unsuitable for IP.
  • Target

    • FunctionPolymerase that creates the 3'-poly(A) tail of mRNA's. Also required for the endoribonucleolytic cleavage reaction at some polyadenylation sites. May acquire specificity through interaction with a cleavage and polyadenylation specificity factor (CPSF) at its C-terminus.
    • Sequence similaritiesBelongs to the poly(A) polymerase family.
    • Post-translational
      Polysumoylated. Varying sumolyation depending on tissue- and cell-type. Highly sumoylated in bladder and NIH 3T3 cells. Sumoylation is required for nuclear localization and enhances PAP stability. Desumoylated by SENP1. Inhibits polymerase activity.
      Hyperphosphorylation on multiple CDK2 consensus and non-consensus sites in the C-terminal Ser/Thr-rich region represses PAP activity in late M-phase. Phosphorylation/dephosphorylation may regulate the interaction between PAP and CPSF.
      Acetylated in the C-terminus. Acetylation decreases interaction with NUDT21 and KPNB1, and inhibits nuclear localization through inhibiting binding to the importin alpha/beta complex.
    • Cellular localizationCytoplasm. Nucleus. The 90 kDa form is nuclear while the 100 kDa and the 106 kDa forms are both nuclear and cytoplasmic.
    • Information by UniProt
    • Database links
    • Alternative names
      • MGC5378 antibody
      • PAP alpha antibody
      • PAP antibody
      • PAP-alpha antibody
      • PapIII antibody
      • PAPOA_HUMAN antibody
      • PAPOLA antibody
      • Plap antibody
      • Poly(A) polymerase alpha antibody
      • Polynucleotide adenylyltransferase alpha antibody
      see all

    Anti-PAPOLA antibody images

    • All lanes : Anti-PAPOLA antibody (ab72492) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg

      Predicted band size : 83 kDa
      Observed band size : 83 kDa

      Exposure time : 3 minutes

    References for Anti-PAPOLA antibody (ab72492)

    ab72492 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for the protocol and result details. Regarding a replacement, there have not been other complaints about the antibody but not many people have purchased it. We have only the lot that you received, and I am concerned that another vial will sim...

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    Thank you for your reply. I have found your order in our system.

    Could you please let me know also the following details:

    1) What antibody dilution and incubation time was used?
    2) What was used for blocking?
    3) You repor...

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