Use at an assay dependent dilution. Detects a band of approximately 500 kDa.
FunctionMetalloproteinase which specifically cleaves IGFBP-4 and IGFBP-5, resulting in release of bound IGF. Cleavage of IGFBP-4 is dramatically enhanced by the presence of IGF, whereas cleavage of IGFBP-5 is slightly inhibited by the presence of IGF.
Tissue specificityHigh levels in placenta and pregnancy serum. In placenta, expressed in X cells in septa and anchoring villi, and in syncytiotrophoblasts in the chorionic villi. Lower levels are found in a variety of other tissues including kidney, myometrium, endometrium, ovaries, breast, prostate, bone marrow, colon, fibroblasts and osteoblasts.
Sequence similaritiesBelongs to the peptidase M43B family. Contains 5 Sushi (CCP/SCR) domains.
Developmental stagePresent in serum and placenta during pregnancy; levels increase throughout pregnancy.
Post-translational modificationsThere appear to be no free sulfhydryl groups.