Overview

  • Product name
  • Description
    Rabbit polyclonal to PAPSS1
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Xenopus laevis, Zebrafish, Xenopus tropicalis
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 347-606 of Human PAPSS1 (Uniprot ID O43252).

  • Positive control
    • Human NCI-N87 xenograft; HeLa cells; Mouse brain and HepG2 whole cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155600 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 71 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate (PAPS: activated sulfate donor used by sulfotransferase). In mammals, PAPS is the sole source of sulfate; APS appears to be only an intermediate in the sulfate-activation pathway. Also involved in the biosynthesis of sulfated L-selectin ligands in endothelial cells.
  • Tissue specificity
    Expressed in testis, pancreas, kidney, thymus, prostate, ovary, small intestine, colon, leukocytes and liver. Also expressed in high endothelial venules (HEV) cells and in cartilage.
  • Pathway
    Sulfur metabolism; sulfate assimilation.
  • Sequence similarities
    In the N-terminal section; belongs to the APS kinase family.
    In the C-terminal section; belongs to the sulfate adenylyltransferase family.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3 prime phosphoadenosine 5 prime phosphosulfate synthase 1 antibody
    • 3' phosphoadenosine 5' phosphosulfate synthase 1 antibody
    • 3''-phosphoadenosine-5''-phosphosulfate synthase antibody
    • Adenosine-5''-phosphosulfate 3''-phosphotransferase antibody
    • Adenylyl-sulfate kinase antibody
    • Adenylylsulfate 3''-phosphotransferase antibody
    • APS kinase antibody
    • ATP-sulfurylase antibody
    • ATPSK 1 antibody
    • ATPSK1 antibody
    • ATPSK2 antibody
    • Bifunctional 3' phosphoadenosine 5' phosphosulfate synthetase 1 antibody
    • PAPS synthase 1 antibody
    • PAPS synthetase 1 antibody
    • PAPS1_HUMAN antibody
    • PAPSS 1 antibody
    • PAPSS antibody
    • PAPSS1 antibody
    • SAT antibody
    • SK 1 antibody
    • SK1 antibody
    • SK2 antibody
    • Sulfate adenylate transferase antibody
    • Sulfurylase kinase 1 antibody
    see all

Images

  • Anti-PAPSS1 antibody (ab155600) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size : 71 kDa
  • Anti-PAPSS1 antibody (ab155600) at 1/500 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size : 71 kDa
  • Immunofluorescence analysis of methanol-fixed HeLa, labeling PAPSS1 with ab155600 at 1/200 dilution. Blue: Hoechst 33342 staining.
  • Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, labeling PAPSS1 with ab155600 at 1/100 dilution.

References

This product has been referenced in:
  • Probert PM  et al. Progenitor-derived hepatocyte-like (B-13/H) cells metabolise 1'-hydroxyestragole to a genotoxic species via a SULT2B1-dependent mechanism. Toxicol Lett 243:98-110 (2016). WB . Read more (PubMed: 26739637) »

See 1 Publication for this product

Customer reviews and Q&As

Filter by Application

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (AR42J-B13)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
AR42J-B13
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
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Submitted Dec 16 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM sodium citrate pH6.0
Permeabilization
No
Specification
Liver
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 20% · Temperature: 21°C
Fixative
Formaldehyde
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Abcam user community

Verified customer

Submitted Dec 16 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate pH6.0
Permeabilization
No
Specification
Liver
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 20% · Temperature: 21°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Dec 16 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (AR42J-B13)
Permeabilization
Yes - Methanol
Specification
AR42J-B13
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 22 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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