Overview

  • Product name
  • Description
    Rabbit polyclonal to PAPSS2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Xenopus laevis
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 1-236 of Human PAPSS2 (UniProt O95340).

  • Positive control
    • H1299 and HeLa cell lysates; Human colon carcinoma tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155588 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 70 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).

Target

  • Relevance
    PAPSS2 is one of the two PAPS synthetases. Three-prime-phosphoadenosine 5-prime-phosphosulfate (PAPS) is the sulfate donor cosubstrate for all sulfotransferase (SULT) enzymes. SULTs catalyze the sulfate conjugation of many endogenous and exogenous compounds, including drugs and other xenobiotics. In humans, PAPS is synthesized from adenosine 5-prime triphosphate (ATP) and inorganic sulfate by 2 isoforms, PAPSS1 and PAPSS2.
  • Database links
  • Alternative names
    • 3 prime phosphoadenosine 5 prime phosphosulfate synthase 2 antibody
    • 3' phosphoadenosine 5' phosphosulfate synthase 2 antibody
    • ATP sulfurylase/adenosine 5' phosphosulfate kinase antibody
    • ATP SULFURYLASE/ADENOSINE 5-PRIME PHOSPHOSULFATE KINASE 2 antibody
    • ATP sulfurylase/APS kinase 2 antibody
    • ATPSK2 antibody
    • Bifunctional 3' phosphoadenosine 5' phosphosulfate synthase 2 antibody
    • Bifunctional 3' phosphoadenosine 5' phosphosulfate synthethase 2 antibody
    • PAPS synthase 2 antibody
    • PAPS synthetase 2 antibody
    • PAPSS 2 antibody
    • PHOSPHOADENOSINE-PHOSPHOSULFATE SYNTHETASE 2 antibody
    • SK 2 antibody
    • SK2 antibody
    • Sulfurylase kinase 2 antibody
    see all

Images

  • Anti-PAPSS2 antibody (ab155588) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size: 70 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling PAPSS2 with ab155588 at 1/200 dilution.

References

This product has been referenced in:
  • Probert PM  et al. Progenitor-derived hepatocyte-like (B-13/H) cells metabolise 1'-hydroxyestragole to a genotoxic species via a SULT2B1-dependent mechanism. Toxicol Lett 243:98-110 (2016). WB . Read more (PubMed: 26739637) »

See 1 Publication for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM sodium citrate pH6.0
Permeabilization
No
Specification
Liver
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 20% · Temperature: 21°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Dec 17 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (Liver)
Gel Running Conditions
Reduced Denaturing (9% gel)
Loading amount
30 µg
Specification
Liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Dec 16 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM sodium citrate pH6.0
Permeabilization
No
Specification
Liver
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 20% · Temperature: 21°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Dec 16 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (AR42J-B13 cell)
Specification
AR42J-B13 cell
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 22 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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