Overview

  • Product name
    Anti-PAQR5 antibody
  • Description
    Rabbit polyclonal to PAQR5
  • Tested applications
    Suitable for: ICC, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Sheep, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide derived from the C terminal of Human PAQR5

  • Positive control
    • Extracts from HUVEC cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab79517 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
ELISA 1/5000.

Target

  • Relevance
    PAQR5 (Progestin and adipoQ receptor family member V) is a steroid membrane receptor which binds progesterone. It may be involved in oocyte maturation. It is an integral membrane protein and is expressed in kidney, colon, adrenal gland and lung. It belongs to the ADIPOR family.
  • Cellular localization
    Membrane; Multi-pass membrane protein.
  • Database links
  • Alternative names
    • FLJ20190 antibody
    • Membrane progestin receptor gamma antibody
    • mPR gamma antibody
    • MPRG antibody
    • PAQR 5 antibody
    • Progestin and adipoQ receptor family member V antibody
    see all

Images

  • All lanes : Anti-PAQR5 antibody (ab79517) at 1/500 dilution

    Lane 1 : Extracts from HUVEC cells
    Lane 2 : Extracts from HUVEC cells with mmunizing peptide at 10 µg

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 38 kDa
    Observed band size : 38 kDa
  • All lanes : Anti-PAQR5 antibody (ab79517) at 500 µg

    Lane 1 : HUVEC cell lysate (positive control)
    Lane 2 : Non-pregnant ovine caruncle tissue lysate
    Lane 3 : Non-pregnant ovine caruncle tissue lysate
    Lane 4 : Non-pregnant ovine caruncle tissue lysate
    Lane 5 : Day 30 of gestation ovine caruncle tissue lysate
    Lane 6 : Day 30 of gestation ovine caruncle tissue lysate
    Lane 7 : Day 30 of gestation ovine caruncle tissue lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    HRP-conjugated goat anti-rabbit IgG monoclonal at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 38 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 52 kDa (possible non-specific binding).

    This image is courtesy of an anonymous Abreview

    See Abreview

  • ab79517 staining PAQR5 in murine ovary tissue by Immunohistochemistry (PFA-fixed).
    Tissue was fixed in paraformaldehyde and blocked using 10% normal goat serum for 1 hour at 25°C, then incubated with ab79517 at a 1/500 dilution for 12 hours at 4°C. The secondary used was a biotin conjugated goat anti-rabbit polyclonal used at a 1/500 dilution.ab79517 staining PAQR5 in murine ovary tissue by Immunohistochemistry (PFA-fixed).

    Tissue was fixed in paraformaldehyde and blocked using 10% normal goat serum for 1 hour at 25°C, then incubated with ab79517 at a 1/500 dilution for 12 hours at 4°C. The secondary used was a biotin conjugated goat anti-rabbit polyclonal used at a 1/500 dilution.

    See Abreview

References

This product has been referenced in:
  • Roche SL  et al. Progesterone Attenuates Microglial-Driven Retinal Degeneration and Stimulates Protective Fractalkine-CX3CR1 Signaling. PLoS One 11:e0165197 (2016). Read more (PubMed: 27814376) »
  • Coleson MP  et al. Human chorionic gonadotropin increases serum progesterone, number of corpora lutea and angiogenic factors in pregnant sheep. Reproduction 150:43-52 (2015). Read more (PubMed: 25861798) »

See all 3 Publications for this product

Customer reviews and Q&As

Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (10 %)
Sample
Sheep Purified protein (caruncle tissue)
Specification
caruncle tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Oct 09 2014

Thank you for contacting Abcam. The immunogen peptide for ab75508 is is from *******and for ab79517, the immnuogen peptide is from ******, both peptides are from the human sequence. If there is anything else I can help you with, please let me know.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Mouse Cell (Mouse ovary sections)
Specification
Mouse ovary sections
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Mr. Nicholas Bashour

Verified customer

Submitted Dec 09 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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