Overview

  • Product nameAnti-PAR4 antibody
    See all PAR4 primary antibodies
  • Description
    Rabbit polyclonal to PAR4
  • Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    A region within synthetic peptide: EPPRTVSGRY KSTTSVSEED VSSRYSRTDR SGFPRYNRDA NVSGTLVSSS, corresponding to internal sequence amino acids 217-266 of Human PAR4

  • Positive control
    • Jurkat cell lysate, human kidney tissue, human liver tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab49155 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/62500.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 37 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • FunctionPro-apoptopic protein capable of selectively inducing apoptosis in cancer cells, sensitizing the cells to diverse apoptotic stimuli and causing regression of tumors in animal models. Induces apoptosis in certain cancer cells by activation of the Fas prodeath pathway and coparallel inhibition of NF-kappa-B transcriptional activity. Inhibits the transcriptional activation and augments the transcriptional repression mediated by WT1. Down-regulates the anti-apoptotic protein BCL2 via its interaction with WT1. Seems also to be a transcriptional repressor by itself. May be directly involved in regulating the amyloid precursor protein (APP) cleavage activity of BACE1.
  • Tissue specificityWidely expressed. Expression is elevated in various neurodegenerative diseases such as amyotrophic lateral sclerosis, Alzheimer, Parkinson and Huntington diseases and stroke. Down-regulated in several cancers.
  • DomainThe leucine-zipper domain is not essential for apoptosis, but is required for sensitization of cells to exogenous apoptotic insults and for interaction with its partners.
    The SAC domain is a death-inducing domain selective for apoptosis induction in cancer cells. This domain is essential for nuclear entry, Fas activation, inhibition of NF-kappa-B activity and induction of apoptosis in cancer cells.
  • Post-translational
    modifications
    Preferentially phosphorylated at the Thr-163 by PKC in cancer cells.
  • Cellular localizationCytoplasm. Nucleus. Mainly cytoplasmic in absence of apoptosis signal and in normal cells. Nuclear in most cancer cell lines. Nuclear entry seems to be essential but not sufficient for apoptosis (By similarity). Nuclear localization includes nucleoplasm and PML nuclear bodies.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2310001G03Rik antibody
    • PAR 4 antibody
    • PAR-4 antibody
    • Pawr antibody
    • PAWR_HUMAN antibody
    • PRKC Apoptosis WT1 Regulator antibody
    • PRKC apoptosis WT1 regulator protein antibody
    • Prostate apoptosis response 4 protein antibody
    • Prostate apoptosis response protein 4 antibody
    • prostate apoptosis response protein PAR-4 antibody
    • Transcriptional repressor Par-4-like protein PAWR antibody
    • Transcriptional repressor PAR4 antibody
    • WT1 Interacting Protein antibody
    see all

Anti-PAR4 antibody images

  • Anti-PAR4 antibody (ab49155) at 2 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG 1/50,000 - 1/100,000

    Predicted band size : 37 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling PAR4 with ab49155 at 4-8µg/ml. Arrows indicate positively labelled eithelial cells of renal tubule. Magnification: 400X.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling PAR4 with ab49155 at 4-8µg/ml. Arrows indicate positively labelled hepatocytes. Magnification: 400X.

References for Anti-PAR4 antibody (ab49155)

ab49155 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"