Anti-PAR4 antibody (ab5787)
Key features and details
- Rabbit polyclonal to PAR4
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-PAR4 antibody
See all PAR4 primary antibodies -
Description
Rabbit polyclonal to PAR4 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Synthetic peptide corresponding to Human PAR4 aa 324-342.
Sequence:EQLKQENKTLLKVVGQLTR
(Peptide available asab5853) -
Positive control
- WB: Rat spleen extract.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab5787 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 2 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 41 kDa).
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Notes |
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WB
Use a concentration of 2 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 41 kDa). |
Target
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Function
Pro-apoptopic protein capable of selectively inducing apoptosis in cancer cells, sensitizing the cells to diverse apoptotic stimuli and causing regression of tumors in animal models. Induces apoptosis in certain cancer cells by activation of the Fas prodeath pathway and coparallel inhibition of NF-kappa-B transcriptional activity. Inhibits the transcriptional activation and augments the transcriptional repression mediated by WT1. Down-regulates the anti-apoptotic protein BCL2 via its interaction with WT1. Seems also to be a transcriptional repressor by itself. May be directly involved in regulating the amyloid precursor protein (APP) cleavage activity of BACE1. -
Tissue specificity
Widely expressed. Expression is elevated in various neurodegenerative diseases such as amyotrophic lateral sclerosis, Alzheimer, Parkinson and Huntington diseases and stroke. Down-regulated in several cancers. -
Domain
The leucine-zipper domain is not essential for apoptosis, but is required for sensitization of cells to exogenous apoptotic insults and for interaction with its partners.
The SAC domain is a death-inducing domain selective for apoptosis induction in cancer cells. This domain is essential for nuclear entry, Fas activation, inhibition of NF-kappa-B activity and induction of apoptosis in cancer cells. -
Post-translational
modificationsPreferentially phosphorylated at the Thr-163 by PKC in cancer cells. -
Cellular localization
Cytoplasm. Nucleus. Mainly cytoplasmic in absence of apoptosis signal and in normal cells. Nuclear in most cancer cell lines. Nuclear entry seems to be essential but not sufficient for apoptosis (By similarity). Nuclear localization includes nucleoplasm and PML nuclear bodies. - Information by UniProt
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Database links
- Entrez Gene: 64513 Rat
- SwissProt: Q62627 Rat
- Unigene: 9127 Rat
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Alternative names
- 2310001G03Rik antibody
- PAR 4 antibody
- PAR-4 antibody
see all
Protocols
Datasheets and documents
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Datasheet download
References (5)
ab5787 has been referenced in 5 publications.
- Guo H et al. PAR-4 overcomes chemo-resistance in breast cancer cells by antagonizing cIAP1. Sci Rep 9:8755 (2019). PubMed: 31217499
- Acharya M et al. A complex regulatory network of transcription factors critical for ocular development and disease. Hum Mol Genet 20:1610-24 (2011). IP . PubMed: 21282189
- Fernandez-Marcos PJ et al. Simultaneous inactivation of Par-4 and PTEN in vivo leads to synergistic NF-kappaB activation and invasive prostate carcinoma. Proc Natl Acad Sci U S A 106:12962-7 (2009). IHC-P ; Human . PubMed: 19470463
- Acharya M et al. Human PRKC apoptosis WT1 regulator is a novel PITX2-interacting protein that regulates PITX2 transcriptional activity in ocular cells. J Biol Chem 284:34829-38 (2009). WB, IHC-P ; Human, Mouse . PubMed: 19801652
- Chung H et al. IGF-I inhibition of apoptosis is associated with decreased expression of prostate apoptosis response-4. J Endocrinol 194:77-85 (2007). PubMed: 17592023