Recombinant
RabMAb

Anti-PAR4 antibody [EPR3991] (ab92590)

Overview

  • Product name
    Anti-PAR4 antibody [EPR3991]
    See all PAR4 primary antibodies
  • Description
    Rabbit monoclonal [EPR3991] to PAR4
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (N terminal)

  • Positive control
    • LNCap, Jurkat, A549 and HeLa cell lysates
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab92590 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notes
    IP: 1/10 - 1/100.
    WB: 1/10000 - 1/50000. Predicted molecular weight: 37 kDa.

    Is unsuitable for Flow Cyt, ICC or IHC-P.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Pro-apoptopic protein capable of selectively inducing apoptosis in cancer cells, sensitizing the cells to diverse apoptotic stimuli and causing regression of tumors in animal models. Induces apoptosis in certain cancer cells by activation of the Fas prodeath pathway and coparallel inhibition of NF-kappa-B transcriptional activity. Inhibits the transcriptional activation and augments the transcriptional repression mediated by WT1. Down-regulates the anti-apoptotic protein BCL2 via its interaction with WT1. Seems also to be a transcriptional repressor by itself. May be directly involved in regulating the amyloid precursor protein (APP) cleavage activity of BACE1.
    • Tissue specificity
      Widely expressed. Expression is elevated in various neurodegenerative diseases such as amyotrophic lateral sclerosis, Alzheimer, Parkinson and Huntington diseases and stroke. Down-regulated in several cancers.
    • Domain
      The leucine-zipper domain is not essential for apoptosis, but is required for sensitization of cells to exogenous apoptotic insults and for interaction with its partners.
      The SAC domain is a death-inducing domain selective for apoptosis induction in cancer cells. This domain is essential for nuclear entry, Fas activation, inhibition of NF-kappa-B activity and induction of apoptosis in cancer cells.
    • Post-translational
      modifications
      Preferentially phosphorylated at the Thr-163 by PKC in cancer cells.
    • Cellular localization
      Cytoplasm. Nucleus. Mainly cytoplasmic in absence of apoptosis signal and in normal cells. Nuclear in most cancer cell lines. Nuclear entry seems to be essential but not sufficient for apoptosis (By similarity). Nuclear localization includes nucleoplasm and PML nuclear bodies.
    • Information by UniProt
    • Database links
    • Alternative names
      • 2310001G03Rik antibody
      • PAR 4 antibody
      • PAR-4 antibody
      • Pawr antibody
      • PAWR_HUMAN antibody
      • PRKC Apoptosis WT1 Regulator antibody
      • PRKC apoptosis WT1 regulator protein antibody
      • Prostate apoptosis response 4 protein antibody
      • Prostate apoptosis response protein 4 antibody
      • prostate apoptosis response protein PAR-4 antibody
      • Transcriptional repressor Par-4-like protein PAWR antibody
      • Transcriptional repressor PAR4 antibody
      • WT1 Interacting Protein antibody
      see all

    Images

    • All lanes : Anti-PAR4 antibody [EPR3991] (ab92590) at 1/10000 dilution

      Lane 1 : LNCap cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : A549 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 37 kDa
    • ab92590 at 1/40 immunoprecipitating PAR4 in Jurkat (human T cell leukemia T lymphocyte) whole cell lysate.

      Lane 1 (+): ab92590 + Jurkat whole cell lysate 

      Lane 2 (-): PBS instead of ab92590 in Jurkat whole cell lysate

      For western blotting, ab92590 was at 1/1000 dilution followed by Goat Anti-Rabbit IgG, (HRP), specific to the non-reduced form of IgG as the secondary antibody (1/1500). 

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    References

    ab92590 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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