The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Adapter protein involved in asymmetrical cell division and cell polarization processes. Probably involved in the formation of epithelial tight junctions. Association with PARD3 may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins.
Expressed in pancreas, skeletal muscle, brain and heart. Weakly expressed in kidney and placenta.
Belongs to the PAR6 family. Contains 1 OPR domain. Contains 1 PDZ (DHR) domain. Contains 1 pseudo-CRIB domain.
The pseudo-CRIB domain together with the PDZ domain is required for the interaction with Rho small GTPases. The OPR domain mediates interactions with MAP2K5. The PDZ domain mediates the interaction with CRB3.
Cytoplasm. Cell membrane. Cell projection > ruffle. Cell junction > tight junction. Colocalizes with GTP-bound CDC42 or RAC1 at membrane ruffles and with PARD3 and PRKCI at epithelial tight junctions.
ab115612, at 5µg/ml, staining PAR6 in Formalin-fixed, Paraffin-embedded Human Heart tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.