Overview

  • Product nameAnti-PAR6 antibody
    See all PAR6 primary antibodies
  • Description
    Goat polyclonal to PAR6
  • Tested applicationsSuitable for: IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide:

    GSRIRGDGSGFSL

    with a Cysteine residue linker, corresponding to C terminal amino acids 334-346 of Human PAR6 (NP_058644.1).

  • Positive control
    • Human Pancreas and Heart tissues.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab115612 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA 1/8000.

Target

  • FunctionAdapter protein involved in asymmetrical cell division and cell polarization processes. Probably involved in the formation of epithelial tight junctions. Association with PARD3 may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins.
  • Tissue specificityExpressed in pancreas, skeletal muscle, brain and heart. Weakly expressed in kidney and placenta.
  • Sequence similaritiesBelongs to the PAR6 family.
    Contains 1 OPR domain.
    Contains 1 PDZ (DHR) domain.
    Contains 1 pseudo-CRIB domain.
  • DomainThe pseudo-CRIB domain together with the PDZ domain is required for the interaction with Rho small GTPases.
    The OPR domain mediates interactions with MAP2K5.
    The PDZ domain mediates the interaction with CRB3.
  • Cellular localizationCytoplasm. Cell membrane. Cell projection > ruffle. Cell junction > tight junction. Colocalizes with GTP-bound CDC42 or RAC1 at membrane ruffles and with PARD3 and PRKCI at epithelial tight junctions.
  • Information by UniProt
  • Database links
  • Alternative names
    • 0710008C04Rik antibody
    • 2610010A15Rik antibody
    • Par 6 partitioning defective 6 C elegans homolog alpha antibody
    • Par 6 partitioning defective 6 homolog alpha antibody
    • Par 6 partitioning defective 6 homolog alpha C elegans antibody
    • PAR 6A antibody
    • PAR-6 alpha antibody
    • PAR-6 antibody
    • par-6 family cell polarity regulator alpha antibody
    • PAR-6A antibody
    • PAR6 antibody
    • PAR6A_HUMAN antibody
    • PAR6alpha antibody
    • PAR6C antibody
    • Pard6a antibody
    • Partitioning defective 6 homolog alpha antibody
    • partitioning-defective protein 6 antibody
    • partitioning-defective protein 6, C. elegans, homolog of, alpha antibody
    • Tax interacting protein 40 antibody
    • Tax interaction protein 40 antibody
    • TAX40 antibody
    • TIP 40 antibody
    • TIP-40 antibody
    • TIP40 antibody
    see all

Anti-PAR6 antibody images

  • ab115612 (10 ug/ml) staining PAR6 in Paraffin-embedded Human Pancreas tissue by Immunihistochemistry ( Microwaved antigen retrieval with Tris/EDTA buffer pH9), HRP staining.
  • ab115612, at 5µg/ml, staining PAR6 in Formalin-fixed, Paraffin-embedded Human Heart tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

References for Anti-PAR6 antibody (ab115612)

ab115612 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"