Anti-Parainfluenza Virus type 1 nucleocapsid antibody [7F5] (ab34752)

Overview

  • Product name
    Anti-Parainfluenza Virus type 1 nucleocapsid antibody [7F5]
  • Description
    Mouse monoclonal [7F5] to Parainfluenza Virus type 1 nucleocapsid
  • Specificity
    This antibody reacts with nucleocapsid protein of human parainfluenza virus type 1 (hPIV-1). It does not cross-react with nucleocapsid proteins of Sendai virus and human parainfluenza virus type 3 (hPIV-3), neither does it react with nucleocapsid proteins of measles, mumps, Menangle, Nipah, and Hendra viruses.
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with human papinfluenza virus. Not yet tested in other species.
  • Immunogen

    Recombinant full length protein corresponding to Parainfluenza Virus type 1 nucleocapsid.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity
    IgG fraction
  • Clonality
    Monoclonal
  • Clone number
    7F5
  • Myeloma
    Sp2/0
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab34752 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000 - 1/10000.
WB 1/1000 - 1/5000. Predicted molecular weight: 58 kDa.

Target

  • Relevance
    Human parainfluenza viruses (HPIV) were first discovered in the late 1950s. HPIV is genetically and antigenically divided into types 1 to 4. HPIV 1 to HPIV 3 are major causes of lower respiratory infections in infants, young children, the immunocompromised, the chronically ill, and the elderly. Each subtype can cause somewhat unique clinical diseases in different hosts. HPIV are enveloped and of medium size (150 to 250 nm), and their RNA genome is in the negative sense. These viruses belong to the Paramyxoviridae family, one of the largest and most rapidly growing groups of viruses causing significant human and veterinary disease. HPIV are closely related to recently discovered megamyxoviruses (Hendra and Nipah viruses) and metapneumovirus. Parainfluenza viruses are enveloped viruses with a negative strand, ~15 500 nucleotide-long nonsegmented RNA genome which encodes two envelope glycoproteins, the haemagglutinin-neuraminidase (HN), and the fusion protein (F, itself cleaved into F1 and F2 subunits), a matrix protein (M), a nucleocapsid protein (N) and several nonstructural proteins including the viral replicase (L).
  • Cellular localization
    Cytoplasmic
  • Alternative names
    • N antibody
    • NP antibody
    • Nucleocapsid protein antibody
    • Nucleoprotein antibody
    • Parainfluenza Nucleoprotein antibody
    see all

Anti-Parainfluenza Virus type 1 nucleocapsid antibody [7F5] images



  • Predicted band size : 58 kDa

    Immunoblot of recombinant nucleocapsid protein (NP) of human parainfluenza virus type 1 (hPIV-1) ab34752 at a concentration of 1 µg/ml.

    MW markers;

    lane 1: crude yeast cell lysate (mock control)

    Lane 2: crude lysate of transformed yeast cells expressing NP of hPIV-1;

    lane 3: purified recombinant NP of hPIV-1;

    lane 4: purified recombinant NP of Sendai virus;

    lane 5: lysate with recombinant NP of hPIV-3;

    lanes 6-8: lysates with recombinant NP of Menangle (6), Hendra (7) and Nipah (8) viruses.

    Panel A : SDS-PAGE, Coomasie staining, B:  immunoblot with ab34752.

References for Anti-Parainfluenza Virus type 1 nucleocapsid antibody [7F5] (ab34752)

ab34752 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Lung)
Permeabilization
Yes - 0.2% Triton X-100
Specification
Lung
Blocking step
Serum as blocking agent for 1 hour(s) and 45 minute(s) · Concentration: 3% · Temperature: 23°C
Fixative
Paraformaldehyde
Username

Christiana Shaw

Verified customer

Submitted Jan 27 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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