Anti-Parainfluenza Virus type 2 antibody (Biotin) (ab20020)

Overview

  • Product name
    Anti-Parainfluenza Virus type 2 antibody (Biotin)
  • Description
    Goat polyclonal to Parainfluenza Virus type 2 (Biotin)
  • Conjugation
    Biotin
  • Specificity
    All structural antigens. Cross reacts with type 2. Minimal reactivity with type 1. Does not react with HEp-2 cells by indirect immunofluorescence. Some cross reactivity with bovine para-3 (<1:400) and canine para (<1,000).
  • Tested applications
    Suitable for: ELISA, ICC/IFmore details
  • Species reactivity
    All structural antigens. Cross reacts with type 2. Minimal reactivity with type 1. Does not react with HEp-2 cells by indirect immunofluorescence. Some cross reactivity with bovine parainfluenza-3 and canine parainfluenza.
  • Immunogen

    Cell preparation: Human isolate, type 3

  • General notes

    Covalently coupled with the N-Hydroxysuccinimide ester of biotin under mild conditions to give a high degree of substitution.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: 10mM PBS, pH 7.2
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    Ab20020 is the IgG fraction of antiserum, purified by sodium sulfate precipitation and ion-exchange chromatography. 4-5mg/ml (OD280nm, E0.1% = 1.4). Titer: >1:1,600 by indirect immunofluorescence
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab20020 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
ICC/IF
  • Application notes
    ELISA: Use at an assay dependent dilution.
    Note: use Avidin and streptavidin amplification system.

    IF: Use at an assay dependent dilution.
    Note: use Avidin and streptavidin amplification system.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Human parainfluenza viruses (HPIV) were first discovered in the late 1950s. Over the last decade considerable knowledge about their molecular structure and function has been accumulated. This has led to significant changes in both the nomenclature and taxonomic relationships of these viruses. HPIV is genetically and antigenically divided into types 1 to 4. HPIV1 to HPIV3 are major causes of lower respiratory infections in infants, young children, the immunocompromised, the chronically ill, and the elderly. Each subtype can cause somewhat unique clinical diseases in different hosts. HPIV are enveloped and of medium size (150 to 250 nm), and their RNA genome is in the negative sense. These viruses belong to the Paramyxoviridae family, one of the largest and most rapidly growing groups of viruses causing significant human and veterinary disease. HPIV are closely related to recently discovered megamyxoviruses (Hendra and Nipah viruses) and metapneumovirus.

    References

    ab20020 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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