Anti-Parathyroid Hormone antibody (ab111097)

Overview

  • Product nameAnti-Parathyroid Hormone antibody
    See all Parathyroid Hormone primary antibodies
  • Description
    Rabbit polyclonal to Parathyroid Hormone
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide, derived from an internal sequence of Human Parathyroid Hormone (UniProt ID: P01270).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferConstituent: Whole serum
  • PurityWhole antiserum
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab111097 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/500.
WB 1/200 - 1/2000. Predicted molecular weight: 13 kDa.

Target

  • FunctionPTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells.
  • Involvement in diseaseDefects in PTH are a cause of familial isolated hypoparathyroidism (FIH) [MIM:146200]; also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. FIH exist both as autosomal dominant and recessive forms of hypoparathyroidism.
  • Sequence similaritiesBelongs to the parathyroid hormone family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • hPTH antibody
    • Parathormone antibody
    • Parathyrin antibody
    • Parathyroid hormone 1 antibody
    • Parathyroid hormone antibody
    • PTH antibody
    • PTH1 antibody
    • PTH1 receptor antibody
    • PTH1R antibody
    • PTHR antibody
    • PTHR1 antibody
    • PTHY_HUMAN antibody
    see all

References for Anti-Parathyroid Hormone antibody (ab111097)

ab111097 has not yet been referenced specifically in any publications.

Product Wall

Thank you very much for your reply and for the information.
I have added a vial of ab40630 to a shipment and forwarded the details of this replacement to your contact.
I will be back in touch regarding ab53040, and if there is anything else th...

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Thank you very much for your reply and for sending the information about the PTHrP expression in A549 cells.
I will follow-up with the lab about ab53040, and I am happy to send a vial of ab40630. I will need to send this vial first to Zotal and th...

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Thank you for your reply.
I can send one of the rabbit polyclonal antibodies ab40630 or ab53040, but their immunogens have lower homology to the rat protein so they may show a weaker cross-reactivity. I certainly understand that a rabbit polyclona...

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Thank you for sending the images and the extra information.
After seeing the images, I agree that the antibody is not binding to the PTH protein as it should be. I would be happy to send a replacement antibody if you would like. We have a few othe...

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Application Western blot
Sample Human Cell lysate - whole cell (transfected HEK293 cells)
Loading amount 250000 cells
Specification transfected HEK293 cells
Gel Running Conditions Reduced Denaturing (12-15%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
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Abcam user community

Verified customer

Submitted Jun 06 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (parathyroid glands)
Loading amount 10 µg
Specification parathyroid glands
Gel Running Conditions Reduced Denaturing (12-15%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted Jun 06 2012

Thank you for taking the time to submit this further information.

I appreciate the time the customer has spent in the laboratory and understand their concerns. It is regrettable the results have not been successful. I would like to make some...

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Thank you for contacting us.

I am sorry to hear your customer has been experiencing problems with one of our Parathyroid Hormone antibodies. The quality of our products is important to us and I would like to reassure you that we investigate ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"