Anti-Parathyroid Hormone antibody (ab40630)

Overview

  • Product nameAnti-Parathyroid Hormone antibody
    See all Parathyroid Hormone primary antibodies
  • Description
    Rabbit polyclonal to Parathyroid Hormone
  • Tested applicationsSuitable for: ELISA, WB, RIA, ICC, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cynomolgus Monkey
  • Immunogen

    Synthetic peptide:

    SVSEIQLMHN LGKHLNSMER VEWLRKKLQD VHNF

    , corresponding to amino acids 32- 66 of Human Parathyroid Hormone covalently attached to a protein carrier.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: None
    Constituents: 0.01% BSA, PBS
  • PurityWhole antiserum
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab40630 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 13 kDa. Blocking with 5% goat or donkey serum significantly reduces background as compared to BSA or milk.
RIA 1/12000.
ICC 1/500.
IHC-FoFr Use at an assay dependent concentration.

Target

  • FunctionPTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells.
  • Involvement in diseaseDefects in PTH are a cause of familial isolated hypoparathyroidism (FIH) [MIM:146200]; also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. FIH exist both as autosomal dominant and recessive forms of hypoparathyroidism.
  • Sequence similaritiesBelongs to the parathyroid hormone family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • hPTH antibody
    • Parathormone antibody
    • Parathyrin antibody
    • Parathyroid hormone 1 antibody
    • Parathyroid hormone antibody
    • PTH antibody
    • PTH1 antibody
    • PTH1 receptor antibody
    • PTH1R antibody
    • PTHR antibody
    • PTHR1 antibody
    • PTHY_HUMAN antibody
    see all

Anti-Parathyroid Hormone antibody images

  • Anti-Parathyroid Hormone antibody (ab40630) at 1 µg/ml + Recombinant Human Parathyroid Hormone protein (ab51234) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 4 minutes

References for Anti-Parathyroid Hormone antibody (ab40630)

ab40630 has not yet been referenced specifically in any publications.

Product Wall



Unfortunately, ab15054 is an unpurified antibody (provided as whole antiserum). We can only provide concentrations for purified antibodies as unpurified antibody preparations vary significantly in specific antibody concentration.

Ne...

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Thank you for your email.

We generally add the location of immunogen e.g. N-terminal, C-terminal or internal on datasheet however I do agree this wasn't the case for this ab53040. This antibody would pick up the whole PTH but not 1-34 aa pepti...

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Thank you for your email.

The immunogen of antibody ab53040 is a sequence from amino acid 71 to aa 100 which might be the reason this antibody is not detecting band of interest.

ab40630 is indeed tested for 100% reactivity with 1-34 p...

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Hier die Antwort aus dem Labor: The carrier protein used was bovine thyroglobulin.

Vielen Dank für Ihre Email.



Bezüglich der Immunogen Sequenz möchte ich bemerken, dass wir immer von der Proprotein Sequenz ausgehen.

Eigentlich sollte das Datenblatt nach 24h aktualisiert gewesen sein... d...

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Vielen Dank für Ihre Anfrage.

Wir haben das Datenblatt nun korrigiert: Es handelt sich wirklich um das reife Protein, nicht um das
Preprohormon.

Die Sequenz lautet: SVSEIQLMHNLGKHLNSMERVEWLRKKLQDVHNF (AS 32-66)

...

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Vielen Dank für Ihre Anfrage.

Ich möchte Sie hiermit wissen lassen, dass ich noch an Ihrer Anfrage arbeite. Ich habe mich mit dem Labor, welches den Antikörper herstellt in Verbindung gesetzt, warte aber immer noch auf eine Antwort. Ich werd...

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Thank you for contacting Abcam about ab40630. I have contacted the laboratory to clarify the concentration of the antibody and they have stated that the product is at 1mg/ml of "neat" rabbit antiserum. If there is anything else I can do to be of assi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"