Ab14496 reacts with parathyroid hormone peptides:aa 15-25; 1-34; 1-38; 1-84; 7-84. There are no cross reactivities with parathyroid hormone peptides: aa 1-3; 1-10; 4-16; 28-48; 39-84; 44-68; 53-84; PTHrP aa 1-
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at a concentration of 1 µg/ml.
IHC-P: Use at a concentration of 2 µg/ml.
IHC-Fr: Use at a concentration of 2 µg/ml.
RIA: Use at a concentration of 25 ng/ml.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells.
Involvement in disease
Defects in PTH are a cause of familial isolated hypoparathyroidism (FIH) [MIM:146200]; also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. FIH exist both as autosomal dominant and recessive forms of hypoparathyroidism.