Anti-Parathyroid Hormone antibody [D1.1] (ab14498)


  • Product nameAnti-Parathyroid Hormone antibody [D1.1]
    See all Parathyroid Hormone primary antibodies
  • Description
    Mouse monoclonal [D1.1] to Parathyroid Hormone
  • Tested applicationsSuitable for: IHC-P, IHC-Fr, ELISAmore details
  • Species reactivity
    Reacts with: Dog, Human
    Predicted to work with: Cow, Cynomolgus Monkey
  • Immunogen

    Synthetic peptide:


    , corresponding to amino acids 53-84 of Human Parathyroid Hormone.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.40
    Constituent: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone numberD1.1
  • IsotypeIgG1
  • Research areas


Our Abpromise guarantee covers the use of ab14498 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2 µg/ml.
IHC-Fr Use at an assay dependent concentration.
ILMA: Use at a concentration of 500 ng/ml.
ELISA Use at an assay dependent concentration. Use at a concentration of 500 ng/ml.


  • FunctionPTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells.
  • Involvement in diseaseDefects in PTH are a cause of familial isolated hypoparathyroidism (FIH) [MIM:146200]; also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. FIH exist both as autosomal dominant and recessive forms of hypoparathyroidism.
  • Sequence similaritiesBelongs to the parathyroid hormone family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • hPTH antibody
    • Parathormone antibody
    • Parathyrin antibody
    • Parathyroid hormone 1 antibody
    • Parathyroid hormone antibody
    • PTH antibody
    • PTH1 antibody
    • PTH1 receptor antibody
    • PTH1R antibody
    • PTHR antibody
    • PTHR1 antibody
    • PTHY_HUMAN antibody
    see all

Anti-Parathyroid Hormone antibody [D1.1] images

  • ab14498 staining Parathyroid Hormone in canine parathyroid tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using the microwave method. Samples were then blocked with 10% serum for 20 minutes at room temperature followed by incubation with the primary antibody at a 1/100 dilution for 50 minutes. An undiluted biotin-conjugated goat anti-mouse polyclonal was used as secondary antibody.

References for Anti-Parathyroid Hormone antibody [D1.1] (ab14498)

ab14498 has not yet been referenced specifically in any publications.

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I'm not sure how the IgG concentration can be specifically measured with a spectrophotometer if there were other proteins in the vial. All proteins (containing aromatic residues) will ...

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I've heard back from the lab, and each new lot of antibody is tested after production (i.e. before lyophilization) using a Bradford assay and then is tested again after lyophiliza...

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Thanks again for all of your patience regarding this issue. I've set up the replacement order, and these vials will arrive on the order *** sometime next week. I will let you know as soon as I have more information from the lab about the QC process for...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Dog Tissue sections (parathyroid)
Specification parathyroid
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Target Retrieval Solution (DAKO) pH 6.0, Microwave 2 x 10 min/700 watt
Permeabilization No
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: RT°C

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Submitted Apr 23 2011