The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIHC-P: 1/50 for 10 min at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionReceptor for parathyroid hormone and for parathyroid hormone-related peptide. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system.
Tissue specificityExpressed in most tissues. Most abundant in kidney, bone and liver.
Involvement in diseaseJansen metaphyseal chondrodysplasia Chondrodysplasia Blomstrand type Enchondromatosis multiple Eiken skeletal dysplasia Primary failure of tooth eruption
Sequence similaritiesBelongs to the G-protein coupled receptor 2 family.