Overview

  • Product name
  • Description
    Rabbit polyclonal to PARC
  • Tested applications
    Suitable for: ICC, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    17 amino acid synthetic peptide, conjugated to KLH, from the C terminal region of Human PARC

  • Positive control
    • Human testis tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab115631 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 281 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Cytoplasmic anchor protein in p53-associated protein complex. Regulates the subcellular localization of p53 and subsequent function. Seems to be part of an atypical cullin-RING-based E3 ubiquitin-protein ligase complex. In vitro, complexes of CUL9/PARC with either CUL7 or TP53 contain E3 ubiquitin-protein ligase activity.
  • Tissue specificity
    Ubiquitously expressed in all tissues with highest expression in testis brain and kidney.
  • Sequence similarities
    Belongs to the cullin family.
    Contains 1 DOC domain.
    Contains 1 IBR-type zinc finger.
    Contains 2 RING-type zinc fingers.
  • Domain
    The IBR domain is required for interaction with UBCH7 and UBCH8.
  • Post-translational
    modifications
    In vitro, self-ubiquitination in the presence of E1, E2 and ubiquitin.
    Neddylated.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • CUL-9 antibody
    • Cul9 antibody
    • CUL9_HUMAN antibody
    • Cullin-9 antibody
    • DKFZp686G1042 antibody
    • DKFZp686P2024 antibody
    • H7AP1 antibody
    • KIAA0708 antibody
    • OTTHUMP00000039816 antibody
    • p53 associated parkin like cytoplasmic protein antibody
    • p53-associated parkin-like cytoplasmic protein antibody
    • PARC antibody
    • parkin-like cytoplasmic p53 binding protein antibody
    • RP3 330M21.2 antibody
    • UbcH 7 associated protein 1 antibody
    • UbcH7 associated protein 1 antibody
    • UbcH7-associated protein 1 antibody
    see all

Images

  • ab115631 at 5ug/ml staining PARC in Human testis tissue by immunohistochemistry (FFPE). Following primary incubation slides were incubated with biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

References

ab115631 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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