The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 281 kDa (predicted molecular weight: 281 kDa).
Use at an assay dependent dilution.
1/2000 - 1/10000.
FunctionCytoplasmic anchor protein in p53-associated protein complex. Regulates the subcellular localization of p53 and subsequent function. Seems to be part of an atypical cullin-RING-based E3 ubiquitin-protein ligase complex. In vitro, complexes of CUL9/PARC with either CUL7 or TP53 contain E3 ubiquitin-protein ligase activity.
Tissue specificityUbiquitously expressed in all tissues with highest expression in testis brain and kidney.
Sequence similaritiesBelongs to the cullin family. Contains 1 DOC domain. Contains 1 IBR-type zinc finger. Contains 2 RING-type zinc fingers.
DomainThe IBR domain is required for interaction with UBCH7 and UBCH8.
Post-translational modificationsIn vitro, self-ubiquitination in the presence of E1, E2 and ubiquitin. Neddylated.