Anti-PARC antibody - Carboxyterminal end (ab62512)

Overview

  • Product nameAnti-PARC antibody - Carboxyterminal end
    See all PARC primary antibodies
  • Description
    Rabbit polyclonal to PARC - Carboxyterminal end
  • Tested applicationsSuitable for: ICC/IF, WB, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (Human) of 17 amino acids from near the C-terminus of PARC

  • Positive control
    • Daudi cells

Properties

Applications

Our Abpromise guarantee covers the use of ab62512 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 281 kDa (predicted molecular weight: 281 kDa).
ICC Use a concentration of 1 µg/ml.

Target

  • FunctionCytoplasmic anchor protein in p53-associated protein complex. Regulates the subcellular localization of p53 and subsequent function. Seems to be part of an atypical cullin-RING-based E3 ubiquitin-protein ligase complex. In vitro, complexes of CUL9/PARC with either CUL7 or TP53 contain E3 ubiquitin-protein ligase activity.
  • Tissue specificityUbiquitously expressed in all tissues with highest expression in testis brain and kidney.
  • Sequence similaritiesBelongs to the cullin family.
    Contains 1 DOC domain.
    Contains 1 IBR-type zinc finger.
    Contains 2 RING-type zinc fingers.
  • DomainThe IBR domain is required for interaction with UBCH7 and UBCH8.
  • Post-translational
    modifications
    In vitro, self-ubiquitination in the presence of E1, E2 and ubiquitin.
    Neddylated.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • CUL-9 antibody
    • Cul9 antibody
    • CUL9_HUMAN antibody
    • Cullin-9 antibody
    • DKFZp686G1042 antibody
    • DKFZp686P2024 antibody
    • H7AP1 antibody
    • KIAA0708 antibody
    • OTTHUMP00000039816 antibody
    • p53 associated parkin like cytoplasmic protein antibody
    • p53-associated parkin-like cytoplasmic protein antibody
    • PARC antibody
    • parkin-like cytoplasmic p53 binding protein antibody
    • RP3 330M21.2 antibody
    • UbcH 7 associated protein 1 antibody
    • UbcH7 associated protein 1 antibody
    • UbcH7-associated protein 1 antibody
    see all

Anti-PARC antibody - Carboxyterminal end images

  • Immunofluorescence of PARC in Daudi cells with ab62512 at 2 ug/mL.

  • Lane 1 : Anti-PARC antibody - Carboxyterminal end (ab62512) at 1 µg/ml
    Lane 2 : Anti-PARC antibody - Carboxyterminal end (ab62512) at 2 µg/ml

    Lane 1 : Daudi lysate
    Lane 2 : Daudi lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 281 kDa
    Observed band size : 281 kDa
  • Immunocytochemistry of Daudi cells labelled with ab62512 at 1 µg/ml.

References for Anti-PARC antibody - Carboxyterminal end (ab62512)

ab62512 has not yet been referenced specifically in any publications.

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