Anti-PARD3 antibody (ab64646)


  • Product nameAnti-PARD3 antibody
    See all PARD3 primary antibodies
  • Description
    Rabbit polyclonal to PARD3
  • Tested applicationsSuitable for: WB, IHC-FrFl, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Sheep, Chimpanzee
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1300 to the C-terminus of Human PARD3.

    (Peptide available as ab73313.)

  • Positive control
    • This antibody gave a positive signal in the following Lysates: HeLa Whole Cell, Rat Thymus Tissue, Rat Brain Tissue, and SW480 Whole Cell This antibody gave a positive result in IHC in the following FFPE tissue: Human lung adenocarcinoma.



Our Abpromise guarantee covers the use of ab64646 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 150 kDa).
IHC-FrFl Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 - 5 µg/ml.
IHC-P Use a concentration of 0.5 µg/ml.


  • FunctionAdapter protein involved in asymmetrical cell division and cell polarization processes. Seems to play a central role in the formation of epithelial tight junctions. Targets the phosphatase PTEN to cell junctions (By similarity). Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons.
  • Tissue specificityWidely expressed.
  • Sequence similaritiesBelongs to the PAR3 family.
    Contains 3 PDZ (DHR) domains.
  • DomainContains a conserved N-terminal oligomerization domain (NTD) that is involved in oligomerization and is essential for proper subapical membrane localization.
    The second PDZ domain mediates interaction with membranes containing phosphoinositol lipids.
  • Post-translational
    Phosphorylated by PRKCZ. EGF-induced Tyr-1127 phosphorylation mediates dissociation from LIMK2.
    Phosphorylation by STK6/AURKA at Ser-962 is required for the normal establishment of neuronal polarity.
  • Cellular localizationEndomembrane system. Cell junction. Cell junction > tight junction. Cell membrane. Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Localized along the cell-cell contact region. Colocalizes with PARD6A and PRKCI at epithelial tight junctions. Colocalizes with the cortical actin that overlays the meiotic spindle during metaphase I and metaphase II (By similarity). Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction.
  • Information by UniProt
  • Database links
  • Alternative names
    • agouti signaling protein antibody
    • ASIP antibody
    • Atypical PKC isotype specific interacting protein antibody
    • Atypical PKC isotype-specific-interacting protein antibody
    • Baz antibody
    • bazooka antibody
    • Coagulation factor II receptor-like 2 antibody
    • CTCL tumor antigen se2-5 antibody
    • F2rl2 antibody
    • PAR-3 antibody
    • par-3 family cell polarity regulator alpha antibody
    • par-3 family cell polarity regulator antibody
    • PAR3 alpha antibody
    • par3 antibody
    • PAR3-alpha antibody
    • PARD-3 antibody
    • Pard3 antibody
    • PARD3_HUMAN antibody
    • PARD3A antibody
    • Partitioning defective 3 homolog antibody
    • Partitioning-defective 3 homolog antibody
    • Partitioning-defective protein 3, C. elegans, homolog of antibody
    • SE2-5L16 antibody
    • SE2-5LT1 antibody
    • SE2-5T2 antibody
    • Thrombin receptor-like 2 antibody
    see all

Anti-PARD3 antibody images

  • ab64646 stained A549 cells. The cells were 100% methanol fixed for 5 minutes at-20°C* and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64646 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • All lanes : Anti-PARD3 antibody (ab64646) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Thymus (Rat) Tissue Lysate
    Lane 3 : Brain (Rat) Tissue Lysate
    Lane 4 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 150 kDa
    Observed band size : 150 kDa
    Additional bands at : 170 kDa,45 kDa,53 kDa,60 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute
  • IHC image of PARD3 staining in Human lung adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64646, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-PARD3 antibody (ab64646)

ab64646 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (duodenum)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: ph9 steamer
Permeabilization No
Specification duodenum
Blocking step BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative Formaldehyde

Miss. Anne Sailer

Verified customer

Submitted Aug 04 2015

Thanks for your enquiry! I will enquire with our supplying lab to determine if ab64840 may also cross react with zebrafish PARD3 and will forward you any information as soon as possible. As we discussed, please reply to this email to let me know if you...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry free floating
Sample Rat Tissue sections (Brain)
Specification Brain

Dr. Sophie Pezet

Verified customer

Submitted Jul 29 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Formaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 27°C

Abcam user community

Verified customer

Submitted Sep 09 2010