Anti-PARD3 antibody [EP310Y] (ab40769)

Overview

  • Product nameAnti-PARD3 antibody [EP310Y]
    See all PARD3 primary antibodies
  • Description
    Rabbit monoclonal [EP310Y] to PARD3
  • SpecificityThis antibody is predicted to detect isoforms 2-7 based on sequence analysis.
  • Tested applicationsSuitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues near the C-terminus of human PARD3. Does not react with PARD 4 or PARD 1.

  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated "PUR" on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab40769 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 150 kDa (predicted molecular weight: 150 kDa).
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notesIs unsuitable for ICC,IHC or IP.
  • Target

    • FunctionAdapter protein involved in asymmetrical cell division and cell polarization processes. Seems to play a central role in the formation of epithelial tight junctions. Targets the phosphatase PTEN to cell junctions (By similarity). Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons.
    • Tissue specificityWidely expressed.
    • Sequence similaritiesBelongs to the PAR3 family.
      Contains 3 PDZ (DHR) domains.
    • DomainContains a conserved N-terminal oligomerization domain (NTD) that is involved in oligomerization and is essential for proper subapical membrane localization.
      The second PDZ domain mediates interaction with membranes containing phosphoinositol lipids.
    • Post-translational
      modifications
      Phosphorylated by PRKCZ. EGF-induced Tyr-1127 phosphorylation mediates dissociation from LIMK2.
      Phosphorylation by STK6/AURKA at Ser-962 is required for the normal establishment of neuronal polarity.
    • Cellular localizationEndomembrane system. Cell junction. Cell junction > tight junction. Cell membrane. Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Localized along the cell-cell contact region. Colocalizes with PARD6A and PRKCI at epithelial tight junctions. Colocalizes with the cortical actin that overlays the meiotic spindle during metaphase I and metaphase II (By similarity). Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction.
    • Information by UniProt
    • Database links
    • Alternative names
      • agouti signaling protein antibody
      • ASIP antibody
      • Atypical PKC isotype specific interacting protein antibody
      • Atypical PKC isotype-specific-interacting protein antibody
      • Baz antibody
      • bazooka antibody
      • Coagulation factor II receptor-like 2 antibody
      • CTCL tumor antigen se2-5 antibody
      • F2rl2 antibody
      • PAR-3 antibody
      • par-3 family cell polarity regulator alpha antibody
      • par-3 family cell polarity regulator antibody
      • PAR3 alpha antibody
      • par3 antibody
      • PAR3-alpha antibody
      • PARD-3 antibody
      • Pard3 antibody
      • PARD3_HUMAN antibody
      • PARD3A antibody
      • Partitioning defective 3 homolog antibody
      • Partitioning-defective 3 homolog antibody
      • Partitioning-defective protein 3, C. elegans, homolog of antibody
      • SE2-5L16 antibody
      • SE2-5LT1 antibody
      • SE2-5T2 antibody
      • Thrombin receptor-like 2 antibody
      see all

    Anti-PARD3 antibody [EP310Y] images

    • All lanes : Anti-PARD3 antibody [EP310Y] (ab40769) at 1/500 dilution

      Lane 1 : LNCap
      Lane 2 : HeLa
      Lane 3 : A431
      Lane 4 : MCF-7

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 150 kDa
      Observed band size : 150 kDa

    References for Anti-PARD3 antibody [EP310Y] (ab40769)

    ab40769 has not yet been referenced specifically in any publications.

    Product Wall

    Based on the sequence alignment, I could not find any information which suggested this antibody would detect PAR4 or 1. I hope this information helps and please don't hesitate to contact us for more advice or information in the future.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"