The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionPutative adapter protein involved in asymmetrical cell division and cell polarization processes. May play a role in the formation of epithelial tight junctions.
Tissue specificityHighly expressed in kidney, lung and skeletal muscle. Expressed at intermediate levels in brain, heart, placenta, liver and pancreas. Isoform 1 is predominant, while isoform 2 and isoform 3 are expressed at lower levels.
Sequence similaritiesBelongs to the PAR3 family. Contains 3 PDZ (DHR) domains.
DomainThe N-terminal part (1-351) part blocks the association of the tight junction marker TJP1 with the cell-cell boundary when it is overexpressed.
Cellular localizationEndomembrane system. Cell junction. Cell junction > tight junction. Partially localized along the cell-cell contact region. Colocalizes with TJP1 to epithelial tight junctions.