Overview

  • Product name
    Anti-PARD3B antibody
  • Description
    Rabbit polyclonal to PARD3B
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence corresponding to amino acids 1036-1141 of Human PARD3B.

  • Positive control
    • Human skin tissue
  • General notes


    ab122264 is mono-specific.

Properties

Applications

Our Abpromise guarantee covers the use of ab122264 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Putative adapter protein involved in asymmetrical cell division and cell polarization processes. May play a role in the formation of epithelial tight junctions.
  • Tissue specificity
    Highly expressed in kidney, lung and skeletal muscle. Expressed at intermediate levels in brain, heart, placenta, liver and pancreas. Isoform 1 is predominant, while isoform 2 and isoform 3 are expressed at lower levels.
  • Sequence similarities
    Belongs to the PAR3 family.
    Contains 3 PDZ (DHR) domains.
  • Domain
    The N-terminal part (1-351) part blocks the association of the tight junction marker TJP1 with the cell-cell boundary when it is overexpressed.
  • Cellular localization
    Endomembrane system. Cell junction. Cell junction > tight junction. Partially localized along the cell-cell contact region. Colocalizes with TJP1 to epithelial tight junctions.
  • Information by UniProt
  • Database links
  • Alternative names
    • ALS2CR19 antibody
    • Amyotrophic lateral sclerosis 2 (juvenile) chromosome region candidate 19 antibody
    • Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 19 protein antibody
    • Par 3 partitioning defective 3 homolog B antibody
    • PAR3-beta antibody
    • PAR3-L protein antibody
    • PAR3B antibody
    • PAR3beta antibody
    • PAR3L antibody
    • PAR3L_HUMAN antibody
    • PAR3LC antibody
    • Pard3b antibody
    • Partitioning defective 3 beta antibody
    • Partitioning defective 3 homolog B antibody
    • Partitioning defective 3-like protein antibody
    see all

Images

  • ab122264, at 1/10 dilution, staining PARD3B in paraffin-embedded Human skin tissue by Immunohistochemistry.

References

ab122264 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Embryonic Skin-Epidermal Keratinocytes in vivo)
Permeabilization
Yes - 0.05% Triton-X 100
Specification
Embryonic Skin-Epidermal Keratinocytes in vivo
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Formaldehyde
Username

Dr. Scott Williams

Verified customer

Submitted Sep 29 2016

Application
Western blot
Sample
Human Cell lysate - whole cell (thyroid cell lines)
Gel Running Conditions
Reduced Denaturing (gel 8%)
Loading amount
25 µg
Specification
thyroid cell lines
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Enke Baldini

Verified customer

Submitted Nov 21 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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