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Western blot - PARK7/DJ1 antibody (ab18257)
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Immunocytochemistry/ Immunofluorescence - PARK7/DJ1 antibody (ab18257)
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Western blot - PARK7/DJ1 antibody (ab18257)
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Product Name
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PARK7/DJ1 antibody
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See all PARK7/DJ1 antibodies (13)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to PARK7/DJ1
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human PARK7/DJ1.
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Reacts with
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Hu, Ms, Rat
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Predicted to react
(species key)
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Cow, Dog
(Due to sequence homology)
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Tested applications
(see key)
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ICC/IF, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 5 µg/ml.
IHC-P: 1/22000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 20 kDa). Can be blocked with PARK7/DJ1 peptide (ab18659).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive control in the following human lysates:
HeLa whole cell
Jurkat whole cell
This antibody gave a positive control in the following mouse lysates:
NIH 3T3 whole cell
Brain tissue
Liver tissue
Heart tissue
Kidney tissue
Pancreas tissue
Testis tissue
Skeletal Muscle tissue
Spinal Cord tissue
Ovary tissue
This antibody gave a positive control in the following rat lysates:
PC12 whole cell
Brain tissue
Liver tissue
Heart tissue
Kidney tissue
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Cellular localization
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Cytoplasmic, Mitochondrial and Nuclear
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Research areas
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Cancer >> Signal transduction >> G protein signaling >> Small G proteins >> Other
Chromatin and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Regulators
Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Parkin / PARK
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Relevance
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PARK7/DJ1 is a ubiquitously expressed protein involved in various cellular processes including cell proliferation, RNA-binding, and oxidative stress. The protein has been found to colocalize within a subset of pathologic tau inclusions in a diverse group of neurodegenerative disorders known as tauopathies (Rizzu et al. 2004). Defects in PARK7/DJ1 are the cause of autosomal recessive early-onset Parkinson's disease 7 (PARK7). Parkinson's disease (PD) is a complex, multifactorial disorder that typically manifests after the age of 50 years. The disease is characterized by bradykinesia, resting tremor, muscular rigidity and postural instability. The pathology involves the loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies (intraneuronal accumulations of aggregated proteins), in surviving neurons in various areas of the brain. PARK7 is characterized by onset before 40 years and slow progression. It has also been suggested that PARK7/DJ1 is a mitogen dependent oncogene product involved in Ras related signal transduction pathways.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Batch dependent within range: 0.40 - 1.00 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this PARK7/DJ1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for PARK7/DJ1 antibody images, references, products related to ab18257 and other tools.
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PARK7/DJ1 antibody images:
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 Immunocytochemistry/ Immunofluorescence - PARK7/DJ1 antibody (ab18257)
ICC/IF image of ab18257 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody
(ab18257, 5µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit
IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking
agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa
Fluor® 594 phalloidin was used to label F-actin (red).
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Western blot - PARK7/DJ1 antibody (ab18257)
All lanes : PARK7/DJ1 antibody (ab18257) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Liver (Mouse) Tissue Lysate - normal tissue
Lane 3 : Heart (Mouse) Tissue Lysate
Lane 4 : Kidney (Mouse) Tissue Lysate
Lane 5 : Pancreas (Mouse) Tissue Lysate (ab29363)
Lane 6 : Testis (Mouse) Tissue Lysate - normal tissue
Lane 7 : Skeletal Muscle (Mouse) Tissue Lysate (ab29711)
Lane 8 : Spinal Cord (Mouse) Tissue Lysate
Lane 9 : Ovary (Mouse) Tissue Lysate - normal tissue
Lane 10 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 11 : Brain (Rat) Tissue Lysate - normal tissue
Lane 12 : Liver (Rat) Tissue Lysate
Lane 13 : Heart (Rat) Tissue Lysate
Lane 14 : Kidney (Rat) Whole Cell Lysate - normal tissue (ab29480)
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 20 kDa
Observed band size : 24 kDa (why is the actual band size different from the predicted?)
Additional bands at : 15 kDa. We are unsure as to the identity of these extra bands.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PARK7/DJ1 antibody (ab18257)
Image courtesy of Human Protein Atlas ab18257 staining PARK7/DJ1 in Human parathyroid. The paraffin embedded tissue was incubated with ab18257 (1/22000 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab18257 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
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Search PubMed (MEDLINE) for references to PARK7/DJ1
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PARK7/DJ1 antibody - more information
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Customer reviews (feedback) regarding PARK7/DJ1 antibody |
Customer FAQs regarding PARK7/DJ1 antibody |
Protocols for PARK7/DJ1 antibody |
Price and availability of products related to PARK7/DJ1 antibody |
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PARK7/DJ1 antibody - related products:
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PARK7/DJ1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about PARK7/DJ1 antibody |
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