PARK7/DJ1 antibody [malphaDJ-1/E2.19] (ab11251)

Product Name 

PARK7/DJ1 antibody [malphaDJ-1/E2.19]

Product type 

Primary antibodies

Description 

Mouse monoclonal [malphaDJ-1/E2.19] to PARK7/DJ1

Immunogen 

Recombinant full length protein (Human).

Reacts with 

Hu, Zfsh

Does not react with 
(species key)

Ms

Specificity 

This clone has been shown to specifically recognise a fusion protein of PARK7/DJ1. It also recognises a FLAG-tagged PARK7/DJ1 expressed in eukaryotic cells.

A single band is seen when Western blotting in optimised conditions with this clone. However, overloading the gel or using low dilutions can cause other bands to appear.

Tested applications 
(see key)

ICC/IF, IHC-FoFr, IHC-Fr, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ICC: Use at an assay dependent dilution.
ICC/IF: 1/500.
IHC-Fr: Use at an assay dependent dilution.
IHC-FoFr: 1/1000.
WB: Use at an assay dependent dilution. Detects a band of approximately 20 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasmic, Mitochondrial and Nuclear

Research areas 

Cancer >> Signal transduction >> G protein signaling >> Small G proteins >> Other
Chromatin and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Regulators
Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Parkin / PARK

Relevance 

PARK7/DJ1 is a ubiquitously expressed protein involved in various cellular processes including cell proliferation, RNA-binding, and oxidative stress. The protein has been found to colocalize within a subset of pathologic tau inclusions in a diverse group of neurodegenerative disorders known as tauopathies (Rizzu et al. 2004). Defects in PARK7/DJ1 are the cause of autosomal recessive early-onset Parkinson's disease 7 (PARK7). Parkinson's disease (PD) is a complex, multifactorial disorder that typically manifests after the age of 50 years. The disease is characterized by bradykinesia, resting tremor, muscular rigidity and postural instability. The pathology involves the loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies (intraneuronal accumulations of aggregated proteins), in surviving neurons in various areas of the brain. PARK7 is characterized by onset before 40 years and slow progression. It has also been suggested that PARK7/DJ1 is a mitogen dependent oncogene product involved in Ras related signal transduction pathways.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

malphaDJ-1/E2.19

Isotype 

IgM

Purity 

Ascites

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for PARK7/DJ1 antibody [malphaDJ-1/E2.19] images, references, products related to ab11251 and other tools.

PARK7/DJ1 antibody [malphaDJ-1/E2.19] images:

References for PARK7/DJ1 antibody [malphaDJ-1/E2.19] (ab11251)

  This product has been used in: (two most recent references)

Melle C et al. Protein profiling of microdissected pancreas carcinoma and identification of HSP27 as a potential serum marker. Clin Chem 53:629-35 (2007). PubMed: 17303689

Bretaud S et al. p53-dependent neuronal cell death in a DJ-1-deficient zebrafish model of Parkinson's disease. J Neurochem 100:1626-35 (2007). PubMed: 17166173

See all 4 publication references for this product.


If you publish research using ab11251 please let us know so that we can cite the reference on this datasheet.

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