Overview

  • Product name
  • Description
    Goat polyclonal to PARK7/DJ1
  • Tested applications
    Suitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human PARK7/DJ1 aa 178-189 (C terminal).
    Sequence:

    C-AAQVKAPLVLKD


    (Peptide available as ab22993)

  • Positive control
    • WB: Jurkat, HeLa and 3T3 whole cell lysate. Human, fetal and adult mouse and rat brain lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab4150 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).Can be blocked with Human PARK7/DJ1 peptide (ab22993).

Target

  • Function
    Protects cells against oxidative stress and cell death. Plays a role in regulating expression or stability of the mitochondrial uncoupling proteins SLC25A14 and SLC25A27 in dopaminergic neurons of the substantia nigra pars compacta and attenuates the oxidative stress induced by calcium entry into the neurons via L-type channels during pacemaking. Eliminates hydrogen peroxide and protects cells against hydrogen peroxide-induced cell death. May act as an atypical peroxiredoxin-like peroxidase that scavenges hydrogen peroxide. Following removal of a C-terminal peptide, displays protease activity and enhanced cytoprotective action against oxidative stress-induced apoptosis. Stabilizes NFE2L2 by preventing its association with KEAP1 and its subsequent ubiquitination. Binds to OTUD7B and inhibits its deubiquitinating activity. Enhances RELA nuclear translocation. Binds to a number of mRNAs containing multiple copies of GG or CC motifs and partially inhibits their translation but dissociates following oxidative stress. Required for correct mitochondrial morphology and function and for autophagy of dysfunctional mitochondria. Regulates astrocyte inflammatory responses. Acts as a positive regulator of androgen receptor-dependent transcription. Prevents aggregation of SNCA. Plays a role in fertilization. Has no proteolytic activity. Has cell-growth promoting activity and transforming activity. May function as a redox-sensitive chaperone.
  • Tissue specificity
    Highly expressed in pancreas, kidney, skeletal muscle, liver, testis and heart. Detected at slightly lower levels in placenta and brain. Detected in astrocytes, Sertoli cells, spermatogonia, spermatids and spermatozoa.
  • Involvement in disease
    Defects in PARK7 are the cause of Parkinson disease type 7 (PARK7) [MIM:606324]. A neurodegenerative disorder characterized by resting tremor, postural tremor, bradykinesia, muscular rigidity, anxiety and psychotic episodes. PARK7 has onset before 40 years, slow progression and initial good response to levodopa. Some patients may show traits reminiscent of amyotrophic lateral sclerosis-parkinsonism/dementia complex (Guam disease).
  • Sequence similarities
    Belongs to the peptidase C56 family.
  • Post-translational
    modifications
    Sumoylated on Lys-130 by PIAS2 or PIAS4; which is enhanced after ultraviolet irradiation and essential for cell-growth promoting activity and transforming activity.
    Cys-106 is easily oxidized to sulfinic acid.
    Undergoes cleavage of a C-terminal peptide and subsequent activation of protease activity in response to oxidative stress.
  • Cellular localization
    Cytoplasm. Nucleus. Mitochondrion. Under normal conditions, located predominantly in the cytoplasm and, to a lesser extent, in the nucleus and mitochondrion. Translocates to the mitochondrion and subsequently to the nucleus in response to oxidative stress and exerts an increased cytoprotective effect against oxidative damage. Detected in tau inclusions in brains from neurodegenerative disease patients.
  • Information by UniProt
  • Database links
  • Alternative names
    • CAP1 antibody
    • DJ-1 antibody
    • DJ1 antibody
    • DJ1 protein antibody
    • Epididymis secretory sperm binding protein Li 67p antibody
    • FLJ27376 antibody
    • FLJ34360 antibody
    • FLJ92274 antibody
    • HEL S 67p antibody
    • Oncogene DJ1 antibody
    • OTTHUMP00000001348 antibody
    • OTTHUMP00000001349 antibody
    • OTTHUMP00000001350 antibody
    • OTTHUMP00000001351 antibody
    • PARK7 antibody
    • PARK7_HUMAN antibody
    • Parkinson disease (autosomal recessive, early onset) 7 antibody
    • Parkinson disease protein 7 antibody
    • Parkinson protein 7 antibody
    • Protein DJ-1 antibody
    • SP22 antibody
    see all

Images

  • All lanes : Anti-PARK7/DJ1 antibody (ab4150) at 0.001 µg/ml

    Lane 1 : HeLa cell lysate (in RIPA buffer)
    Lane 2 : Jurkat cell lysate (in RIPA buffer)

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique

    Predicted band size : 20 kDa
    Observed band size : 24 kDa (why is the actual band size different from the predicted?)

    Primary incubation was 1 hour.

  • All lanes : Anti-PARK7/DJ1 antibody (ab4150) at 0.001 µg/ml

    Lane 1 : Human cerebellum lysate (in RIPA buffer)
    Lane 2 : Human frontal cortex lysate (in RIPA buffer)
    Lane 3 : Human hippocampus lysate (in RIPA buffer)
    Lane 4 : Mouse fetal brain lysate (in RIPA buffer)
    Lane 5 : Mouse brain lysate (in RIPA buffer)
    Lane 6 : Rat brain lysate (in RIPA buffer)

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique

    Predicted band size : 20 kDa
    Observed band size : 24 kDa (why is the actual band size different from the predicted?)

    Primary incubation was 1 hour.



  • Predicted band size : 20 kDa

    Western blot using ab4150 at 1/500.
    Exposure time: 30s.

    Left hand lane: HeLa whole cell lysate (human)
    Right hand lane: 3T3 whole cell lysate (mouse)

  • Anti-PARK7/DJ1 antibody (ab4150) at 1/6000 dilution + Mouse brain tissue lysate - whole at 5 µg

    Secondary
    HRP-conjugated Donkey anti-mouse IgG at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 20 kDa
    Observed band size : 24 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    See Abreview

References

This product has been referenced in:
  • Johnson WM  et al. Regulation of DJ-1 by Glutaredoxin 1 in Vivo: Implications for Parkinson's Disease. Biochemistry 55:4519-32 (2016). WB ; Mouse . Read more (PubMed: 26894491) »
  • Piroli GG  et al. Succination is Increased on Select Proteins in the Brainstem of the NADH dehydrogenase (ubiquinone) Fe-S protein 4 (Ndufs4) Knockout Mouse, a Model of Leigh Syndrome. Mol Cell Proteomics 15:445-61 (2016). Read more (PubMed: 26450614) »

See all 15 Publications for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Medaka fish Tissue lysate - whole (whole brain)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
5 µg
Specification
whole brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Tyrone Genade

Verified customer

Submitted Sep 21 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Nothobranchius furzeri Tissue lysate - whole (whole brain)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
5 µg
Specification
whole brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Tyrone Genade

Verified customer

Submitted Sep 21 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Zebrafish Tissue lysate - whole (whole brain)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
5 µg
Specification
whole brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Tyrone Genade

Verified customer

Submitted Sep 21 2016

Application
Western blot
Sample
Rat Tissue lysate - whole (midbrain)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
10 µg
Specification
midbrain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Tyrone Genade

Verified customer

Submitted Sep 06 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Brain)
Specification
Brain
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization
No
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted Sep 16 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Tissue lysate - whole (Brain cortex)
Total protein in input
250 µg
Specification
Brain cortex
Immuno-precipitation step
Protein G
Username

Abcam user community

Verified customer

Submitted Sep 09 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Specification
Brain
Gel Running Conditions
Reduced Denaturing (10% tris-glycine)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted Sep 07 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (Brain cortex)
Loading amount
10 µg
Specification
Brain cortex
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted Apr 07 2009

Thank you for your enquiry. Yes, this antibody has been tested for cross-reactivity with both mouse and human. On the online datasheet for ab4150 there are two Western blot images, the first one using Jurkat lysate and the second one showing HeLa whole...

Read More

I'm sorry to hear you are having a problem with our PARK7 (DJ1) antibody ab4150. I would like to suggest the following modifications to your protocol: - The protein subcellular localisation is described as both nuclear and cytoplasmic, I would th...

Read More

1-10 of 12 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up