Overview

  • Product nameAnti-PARK7/DJ1 antibody
    See all PARK7/DJ1 primary antibodies
  • Description
    Goat polyclonal to PARK7/DJ1
  • Tested applicationsIP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide:

    C-AAQVKAPLVLKD

    , corresponding to C terminal amino acids 178-189 of Human PARK7/DJ1 (Peptide available as ab22993.)

  • Positive control
    • WB: Jurkat lysate, HeLa whole cell lysate , 3T3 whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab4150 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).Can be blocked with Human PARK7/DJ1 peptide (ab22993). Use at a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa). Use RIPA extraction buffer.

Target

  • FunctionProtects cells against oxidative stress and cell death. Plays a role in regulating expression or stability of the mitochondrial uncoupling proteins SLC25A14 and SLC25A27 in dopaminergic neurons of the substantia nigra pars compacta and attenuates the oxidative stress induced by calcium entry into the neurons via L-type channels during pacemaking. Eliminates hydrogen peroxide and protects cells against hydrogen peroxide-induced cell death. May act as an atypical peroxiredoxin-like peroxidase that scavenges hydrogen peroxide. Following removal of a C-terminal peptide, displays protease activity and enhanced cytoprotective action against oxidative stress-induced apoptosis. Stabilizes NFE2L2 by preventing its association with KEAP1 and its subsequent ubiquitination. Binds to OTUD7B and inhibits its deubiquitinating activity. Enhances RELA nuclear translocation. Binds to a number of mRNAs containing multiple copies of GG or CC motifs and partially inhibits their translation but dissociates following oxidative stress. Required for correct mitochondrial morphology and function and for autophagy of dysfunctional mitochondria. Regulates astrocyte inflammatory responses. Acts as a positive regulator of androgen receptor-dependent transcription. Prevents aggregation of SNCA. Plays a role in fertilization. Has no proteolytic activity. Has cell-growth promoting activity and transforming activity. May function as a redox-sensitive chaperone.
  • Tissue specificityHighly expressed in pancreas, kidney, skeletal muscle, liver, testis and heart. Detected at slightly lower levels in placenta and brain. Detected in astrocytes, Sertoli cells, spermatogonia, spermatids and spermatozoa.
  • Involvement in diseaseDefects in PARK7 are the cause of Parkinson disease type 7 (PARK7) [MIM:606324]. A neurodegenerative disorder characterized by resting tremor, postural tremor, bradykinesia, muscular rigidity, anxiety and psychotic episodes. PARK7 has onset before 40 years, slow progression and initial good response to levodopa. Some patients may show traits reminiscent of amyotrophic lateral sclerosis-parkinsonism/dementia complex (Guam disease).
  • Sequence similaritiesBelongs to the peptidase C56 family.
  • Post-translational
    modifications
    Sumoylated on Lys-130 by PIAS2 or PIAS4; which is enhanced after ultraviolet irradiation and essential for cell-growth promoting activity and transforming activity.
    Cys-106 is easily oxidized to sulfinic acid.
    Undergoes cleavage of a C-terminal peptide and subsequent activation of protease activity in response to oxidative stress.
  • Cellular localizationCytoplasm. Nucleus. Mitochondrion. Under normal conditions, located predominantly in the cytoplasm and, to a lesser extent, in the nucleus and mitochondrion. Translocates to the mitochondrion and subsequently to the nucleus in response to oxidative stress and exerts an increased cytoprotective effect against oxidative damage. Detected in tau inclusions in brains from neurodegenerative disease patients.
  • Information by UniProt
  • Database links
  • Alternative names
    • CAP1 antibody
    • DJ 1 antibody
    • DJ-1 antibody
    • DJ1 antibody
    • DJ1 protein antibody
    • DJ1 protein antibody
    • FLJ27376 antibody
    • FLJ34360 antibody
    • FLJ92274 antibody
    • Oncogene DJ1 antibody
    • OTTHUMP00000001348 antibody
    • OTTHUMP00000001349 antibody
    • OTTHUMP00000001350 antibody
    • OTTHUMP00000001351 antibody
    • Park 7 antibody
    • Park7 antibody
    • PARK7_HUMAN antibody
    • Parkinson disease (autosomal recessive early onset) 7 antibody
    • Parkinson disease (autosomal recessive, early onset) 7 antibody
    • Parkinson disease protein 7 antibody
    • Parkinson protein 7 antibody
    • Protein DJ 1 antibody
    • Protein DJ-1 antibody
    • RNA binding protein regulatory subunit antibody
    • RS antibody
    • SP22 antibody
    see all

Anti-PARK7/DJ1 antibody images



  • Predicted band size : 20 kDa


    ab4150 staining (1µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.


  • Predicted band size : 20 kDa


    Western blot using ab4150 at 1/500.
    Exposure time: 30s.

    Left hand lane: HeLa whole cell lysate (human)
    Right hand lane: 3T3 whole cell lysate (mouse)
  • Anti-PARK7/DJ1 antibody (ab4150) at 1/6000 dilution

    Secondary
    HRP conjugated Donkey anti mouse at 1/10000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 20 kDa
    Observed band size : 24 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Anti-PARK7/DJ1 antibody (ab4150)

This product has been referenced in:
  • Cornelius N  et al. Cellular consequences of oxidative stress in riboflavin responsive multiple acyl-CoA dehydrogenation deficiency patient fibroblasts. Hum Mol Genet N/A:N/A (2014). Read more (PubMed: 24698980) »
  • Miyama A  et al. Oxidation of DJ-1 Induced by 6-Hydroxydopamine Decreasing Intracellular Glutathione. PLoS One 6:e27883 (2011). WB ; Human . Read more (PubMed: 22132160) »

See all 8 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Brain)
Specification Brain
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization No
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
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Abcam user community

Verified customer

Submitted Sep 16 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Tissue lysate - whole (Brain cortex)
Total protein in input 250 µg
Specification Brain cortex
Immuno-precipitation step Protein G
Username

Abcam user community

Verified customer

Submitted Sep 09 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Brain)
Specification Brain
Gel Running Conditions Reduced Denaturing (10% tris-glycine)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted Sep 07 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (Brain cortex)
Loading amount 10 µg
Specification Brain cortex
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted Apr 07 2009

Thank you for your enquiry. Yes, this antibody has been tested for cross-reactivity with both mouse and human. On the online datasheet for ab4150 there are two Western blot images, the first one using Jurkat lysate and the second one showing HeLa whole...

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I'm sorry to hear you are having a problem with our PARK7 (DJ1) antibody ab4150. I would like to suggest the following modifications to your protocol: - The protein subcellular localisation is described as both nuclear and cytoplasmic, I would th...

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Thank you for your enquiry and patience. It is interesting that you got a single band at 28 kDa. The predicted molecular weight of Park7/DJ-1 is 19.8 kDa (Swissprot Accession Number Q99497) and an approximately 20 kDa band was seen in Jurkat lysate whe...

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Thank you for your enquiry. You raise an interesting point and we have taken it very seriously. As you may know the predicted molecular weight of Park7/DJ-1 is 19.8 kDa (Swissprot Accession Number Q99497) so a band of 28 kDa is substantially larger t...

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