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Recombinant full length protein (Human).
This monoclonal antibody to DJ-1 has been knockout validated in Western blot. The expected band for DJ-1 was observed in wild type cells and the band was not seen in knockout cells.
Our Abpromise guarantee covers the use of ab11251 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/100. ab91545-Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.|
|IHC-Fr||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 20 kDa.|
|ICC||Use at an assay dependent concentration.|
Western blot using ab11251 at 1/500 on HeLa whole cell lysate (20
Western blot using clone malphaDJ-1/E2.19 and a beta actin antibody as a loading control.
The bottom band is PARK7/DJ1, the top band is beta actin.
Lane 1: 293 cell lysate
Lane 2: MCF-7 cell lysate
Lanes 3-7: various different prostate cell lines
Lane 8: recombinant PARK7/DJ1 (that was used as immunogen for this antibody)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: PARK7/DJ1 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Human brain tissue lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab11251 observed at 24 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab11251 detected the expected band for DJ-1 in wild type cells and the band was not seen in knockout cells. Wild-type and PARK7/DJ1 knockout samples were subjected to SDS-PAGE. ab11251 and ab181602 (loading control to GAPDH) were diluted at 1/500 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"