Overview

  • Product name
    Anti-PARM-1 antibody [EPR10009]
    See all PARM-1 primary antibodies
  • Description
    Rabbit monoclonal [EPR10009] to PARM-1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human PARM-1 (UniProt ID: Q6UWI2).

  • Positive control
    • Human placenta, fetal kidney, fetal heart, and 293T lysates; permeabilized 293 cells; 293 cell lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR10009
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab168369 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 32 kDa.
IP 1/100 - 1/500.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC or IHC-P.
  • Target

    Images

    • All lanes : Anti-PARM-1 antibody [EPR10009] (ab168369) at 1/1000 dilution

      Lane 1 : Human placenta lysate
      Lanes 2-3 : fetal kidney lysate
      Lane 4 : 293T lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 32 kDa

    • Western blot analysis on pellet from 293 cell lysate immunoprecipitated using ab168369 at a 1/100 dilution, and HRP-conjugated anti-rabbit IgG.
    • Flow cytometric analysis of permeabilized 293 cells using ab168369 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).

    References

    This product has been referenced in:
    • Lee M  et al. Transcriptional programs of lymphoid tissue capillary and high endothelium reveal control mechanisms for lymphocyte homing. Nat Immunol 15:982-95 (2014). Read more (PubMed: 25173345) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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