Overview

  • Product name
  • Description
    Rabbit polyclonal to PARN
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 589-639 of Human PARN (NP_002573.1).

  • Positive control
    • HeLa, Jurkat and 293T whole cell lysates

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7 to 8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab125185 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 73 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function
    3'-exoribonuclease that has a preference for poly(A) tails of mRNAs, thereby efficiently degrading poly(A) tails. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs and is also used to silence certain maternal mRNAs translationally during oocyte maturation and early embryonic development. Interacts with both the 3'-end poly(A) tail and the 5'-end cap structure during degradation, the interaction with the cap structure being required for an efficient degradation of poly(A) tails. Involved in nonsense-mediated mRNA decay, a critical process of selective degradation of mRNAs that contain premature stop codons. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly via its interaction with KHSRP. Probably mediates the removal of poly(A) tails of AREs mRNAs, which constitutes the first step of destabilization.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the CAF1 family.
    Contains 1 R3H domain.
  • Cellular localization
    Nucleus. Cytoplasm. Nucleus > nucleolus. Some nuclear fraction is nucleolar.
  • Information by UniProt
  • Database links
  • Alternative names
    • DAN antibody
    • Deadenylating nuclease antibody
    • Deadenylation nuclease antibody
    • PARN antibody
    • PARN_HUMAN antibody
    • Poly A specific ribonuclease antibody
    • Poly(A) specific ribonuclease antibody
    • Poly(A)-specific ribonuclease PARN antibody
    • Polyadenylate specific ribonuclease antibody
    • Polyadenylate-specific ribonuclease antibody
    see all

Images

  • All lanes : Anti-PARN antibody (ab125185) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 73 kDa


    Exposure time : 3 minutes
  • ab125185, at 6 µg/mg lysate, detecting PARN in HeLa cell lysate (1 mg of immunoprecipitate, 20% loaded in the lane) by Immunoprecipitation.
    For the subsequent Western blot, ab125185 was used at 1 µg/ml.
    The blot was developed using the ECL technique, with an exposure time of 3 seconds.

References

ab125185 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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