• Product nameAnti-PARN antibody
    See all PARN primary antibodies
  • Description
    Rabbit polyclonal to PARN
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 500 - 600 of Human PARN.

    (Peptide available as ab28513.)

  • Positive control
    • This antibody gave a positive signal in HeLa, Jurkat and A431 whole cell lysates


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab27778 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesICC/IF: 1/100 (please see abreview).
    IHC-P: 1/25. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    WB: Use at a concentration of 1 - 5 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 73 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function3'-exoribonuclease that has a preference for poly(A) tails of mRNAs, thereby efficiently degrading poly(A) tails. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs and is also used to silence certain maternal mRNAs translationally during oocyte maturation and early embryonic development. Interacts with both the 3'-end poly(A) tail and the 5'-end cap structure during degradation, the interaction with the cap structure being required for an efficient degradation of poly(A) tails. Involved in nonsense-mediated mRNA decay, a critical process of selective degradation of mRNAs that contain premature stop codons. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly via its interaction with KHSRP. Probably mediates the removal of poly(A) tails of AREs mRNAs, which constitutes the first step of destabilization.
    • Tissue specificityUbiquitous.
    • Sequence similaritiesBelongs to the CAF1 family.
      Contains 1 R3H domain.
    • Cellular localizationNucleus. Cytoplasm. Nucleus > nucleolus. Some nuclear fraction is nucleolar.
    • Information by UniProt
    • Database links
    • Alternative names
      • DAN antibody
      • Deadenylating nuclease antibody
      • Deadenylation nuclease antibody
      • PARN antibody
      • PARN_HUMAN antibody
      • Poly A specific ribonuclease antibody
      • Poly(A) specific ribonuclease antibody
      • Poly(A)-specific ribonuclease PARN antibody
      • Polyadenylate specific ribonuclease antibody
      • Polyadenylate-specific ribonuclease antibody
      see all

    Anti-PARN antibody images

    • All lanes : Anti-PARN antibody (ab27778) at 1 µg/ml

      Lane 1 : HeLa Whole Cell Lysate
      Lane 2 : Jurkat whole cell lysate (ab7899)
      Lane 3 : A431 whole cell lysate (ab7909)

      Lysates/proteins at 20 µg per lane.

      Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size : 73 kDa
    • ab27778, at 1/100 dilution, staining human PARN in HeLa cells.

      See Abreview

    • Image courtesy of Human Protein Atlas

      ab27778 staining PARN in human small intestine. Paraffin embedded human small intestine tissue was incubated with ab27778 (1/25 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

      ab27778 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.

      Further results for this antibody can be found at www.proteinatlas.org

    References for Anti-PARN antibody (ab27778)

    ab27778 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Fixative Paraformaldehyde

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Dec 18 2006