Overview

  • Product nameAnti-PARP antibody [A6.4.12]
    See all PARP primary antibodies
  • Description
    Mouse monoclonal [A6.4.12] to PARP
  • Tested applicationsSuitable for: WB, IP, ELISA, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Hamster, Xenopus laevis, Drosophila melanogaster
  • Immunogen

    Native Human PARP protein.

  • Positive control
    • Human placenta, skin and spleen tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab110915 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 113 kDa.
IP Use at an assay dependent concentration.
ELISA 1/1000 - 1/5000.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.

Target

  • FunctionInvolved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150.
  • Sequence similaritiesContains 1 BRCT domain.
    Contains 1 PARP alpha-helical domain.
    Contains 1 PARP catalytic domain.
    Contains 2 PARP-type zinc fingers.
  • Post-translational
    modifications
    Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.
    Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
    S-nitrosylated, leading to inhibit transcription regulation activity.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase) antibody
    • ADP ribosyltransferase diphtheria toxin like 1 antibody
    • ADP ribosyltransferase NAD(+) antibody
    • ADPRT 1 antibody
    • ADPRT antibody
    • ADPRT1 antibody
    • ARTD1 antibody
    • msPARP antibody
    • NAD(+) ADP ribosyltransferase 1 antibody
    • NAD(+) ADP-ribosyltransferase 1 antibody
    • pADPRT 1 antibody
    • pADPRT1 antibody
    • PARP 1 antibody
    • PARP antibody
    • PARP-1 antibody
    • PARP1 antibody
    • PARP1_HUMAN antibody
    • Poly (ADP ribose) polymerase 1 antibody
    • poly (ADP ribose) polymerase family, member 1 antibody
    • Poly [ADP-ribose] polymerase 1 antibody
    • Poly(ADP ribose) polymerase antibody
    • poly(ADP ribose) synthetase antibody
    • poly(ADP ribosyl)transferase antibody
    • Poly[ADP ribose] synthetase 1 antibody
    • Poly[ADP-ribose] synthase 1 antibody
    • PPOL antibody
    see all

Anti-PARP antibody [A6.4.12] images

  • ab110915, at 10 µg/ml, staining PARP in formalin fixed, paraffin embedded Human placenta by Immunohistochemistry. Biotinylated anti mouse IgG secondary antibody, alkaline phosphatase streptavidin and chromogen was used to develop staining.
  • ab110915, at 10 µg/ml, staining PARP in formalin fixed, paraffin embedded Human skin by Immunohistochemistry. Biotinylated anti mouse IgG secondary antibody, alkaline phosphatase streptavidin and chromogen was used to develop staining.
  • ab110915, at 10 µg/ml, staining PARP in formalin fixed, paraffin embedded Human spleen by Immunohistochemistry. Biotinylated anti mouse IgG secondary antibody, alkaline phosphatase streptavidin and chromogen was used to develop staining.

References for Anti-PARP antibody [A6.4.12] (ab110915)

This product has been referenced in:
  • Wysham WZ  et al. BRCAness profile of sporadic ovarian cancer predicts disease recurrence. PLoS One 7:e30042 (2012). IHC-P ; Human . Read more (PubMed: 22253870) »

See 1 Publication for this product

Product Wall

Thank you for contacting us. We do have positive controls available for these antibodies. The Jurkat lysate catalog number is ab7899. This should also be an appropriate control for the anti-RIP3 antibody, as we do not have the HEK293 transfected cells ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"