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Western blot - PARP antibody (ab6079)
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Western blot - PARP antibody (ab6079)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-PARP antibody(ab6079)
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Product Name
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PARP antibody
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See all PARP antibodies (12)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to PARP
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Immunogen
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Synthetic peptide mapping to the amino terminal domain of human PARP.
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Reacts with
(species key)
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Hu, Ms, Rat
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Tested applications
(see key)
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IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: 1/400. Reactive with the 112 KD and 29 KD fragments.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Jurkat whole cell lysate
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> DNA Damage Recognition
Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> ADP-ribosylation
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Relevance
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PARP (Poly (ADP-ribose) polymerase) is a 113 kDa nuclear protein which can exist as a homo- or hetero-dimer, and is strongly activated by DNA strand breaks. This protein acts as a molecular “nick sensor” and functions in base excision repair, poly(ADPribosyl)ation of acceptor proteins involved in chromatin architecture and DNA metabolism, and participates in protein modification to enhance or repress transcription. PARP also plays a role in other cellular processes, including cell proliferation and differentiation. PARP is ribosylated by PARP2, and during apoptosis, ICE family members, such as caspase 3 and 7, cleave PARP to yield an 85 kDa and a 25 kDa fragment. PARP cleavage is considered to be one of the classical characteristics of apoptosis. PARP interacts with proteins in the base excision repair complex containing at least XRCC1, PARP2, POLB and LIG3. In addition PARP forms heterodimers with PARP2, and interacts with PARP3.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: 0.2% Gelatin, PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.200 mg/ml
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Storage instructions
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Store at +4°C. Do not freeze.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this PARP antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for PARP antibody images, references, products related to ab6079 and other tools.
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PARP antibody images:
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 Western blot - PARP antibody (ab6079)
All lanes : PARP antibody (ab6079) at 1/1000 dilution
Lane 1 : 15ug mouse MEF cell lysate (untreated)
Lane 2 : 15ug mouse MEF cell lysate: cells treated for 1 hour with adriamycin
Lane 3 : 15ug mouse MEF cell lysate: cells treated for 8 hours with adriamycin
Secondary
HRP conjugated donkey anti-rabbit polyclonal antibody
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 122 kDa
Observed band size : 113 kDa (why is the actual band size different from the predicted?)
Exposure time : 1 minute
This image is courtesy of an Abreview submitted by Zeng Li.
See Abreview
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Western blot - PARP antibody (ab6079)
All lanes : PARP antibody (ab6079) at 1/250 dilution
Lane 1 : 30ug untreated HeLa whole cell lysate
Lane 2 : 30ug HeLa whole cell lysate treated with 1 uM Staurosporine overnight
Secondary
HRP conjugated Goat anti-rabbit
Predicted band size : 122 kDa
Observed band size : 29 kDa (why is the actual band size different from the predicted?)
This image is courtesy of an Abreview submitted by Dr Jerome Lemonnier
See Abreview
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-PARP antibody(ab6079)
Ab6079 staining Human temporal cortex. Staining is localized to the nucleus.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
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References for PARP antibody (ab6079)
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This product has been used in: (two most recent references)
Podowski M et al. Complex integration of matrix, oxidative stress, and apoptosis in genetic emphysema. Am J Pathol 175:84-96 (2009). WB; Mouse. PubMed: 19541933
Tasatargil A et al. Aldosterone-induced endothelial dysfunction of rat aorta: role of poly(ADP-ribose) activation. J Renin Angiotensin Aldosterone Syst 10:127-37 (2009). IHC-P; Rat. PubMed: 19713411
See all 3 publication references for this product.
If you publish research using ab6079 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to PARP
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PARP antibody - more information
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Customer reviews (feedback) regarding PARP antibody |
Customer FAQs regarding PARP antibody |
Protocols for PARP antibody |
Price and availability of products related to PARP antibody |
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PARP antibody - related products:
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PARP antibody - other tools:
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Contact Abcam with a Technical Enquiry about PARP antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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