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Overview
Product name
Anti-PARP antibody
See all PARP products (13) ...
Description
Rabbit polyclonal to PARP
Tested applications
ICC/IF, WB, IP, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide mapping to the amino terminal domain of human PARP.
Positive control
Jurkat whole cell lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 0.2% Gelatin, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> DNA Damage Recognition
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Base Excision Repair
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> ADP-ribosylation
Applications
Show applications key
Our Abpromise guarantee covers the use of ab6079 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: 1/500
WB: 1/400Detects a band of approximately 112 , 29 kDa (predicted molecular weight: 122 kDa).
IP: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent dilution.
Target
Function
Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150.
Sequence similarities
Contains 1 BRCT domain.
Contains 1 PARP alpha-helical domain.
Contains 1 PARP catalytic domain.
Contains 2 PARP-type zinc fingers.
Post-translational
modifications
Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.
Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
S-nitrosylated, leading to inhibit transcription regulation activity.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP09874
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase) antibody
- ADP ribosyltransferase NAD+ antibody
- ADPRT 1 antibody
see all
Database links
- Entrez Gene: 142 Human
- Entrez Gene: 11545 Mouse
- Entrez Gene: 25591 Rat
- Omim: 173870 Human
- SwissProt: P09874 Human
- SwissProt: P11103 Mouse
- SwissProt: P27008 Rat
- Unigene: 177766 Human
see all
Anti-PARP antibody images:
Western blot - PARP antibody (ab6079)
All lanes : Anti-PARP antibody (ab6079) at 1/250 dilution
Lane 1 : 30ug untreated HeLa whole cell lysate
Lane 2 : 30ug HeLa whole cell lysate treated with 1 uM Staurosporine overnight
Secondary
HRP conjugated Goat anti-rabbit
Predicted band size : 122 kDa
Observed band size : 29 kDa (why is the actual band size different from the predicted?)
This image is courtesy of an Abreview submitted by Dr Jerome Lemonnier
Immunocytochemistry/ Immunofluorescence - PARP antibody (ab6079)
ab6079 (1/500) staining PARP in assynchronous HeLa cells (green). Cells were fixed with paraformaldehyde, permeabilised with 0.5% Triton X-100 and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please see abreview.
Image part of an Abreview submitted by Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canada
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-PARP antibody(ab6079)
Ab6079 staining Human temporal cortex. Staining is localized to the nucleus.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Western blot - PARP antibody (ab6079)
All lanes : Anti-PARP antibody (ab6079) at 1/1000 dilution
Lane 1 : 15ug mouse MEF cell lysate (untreated)
Lane 2 : 15ug mouse MEF cell lysate: cells treated for 1 hour with adriamycin
Lane 3 : 15ug mouse MEF cell lysate: cells treated for 8 hours with adriamycin
Secondary
HRP conjugated donkey anti-rabbit polyclonal antibody
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 122 kDa
Observed band size : 113 kDa (why is the actual band size different from the predicted?)
Exposure time : 1 minute
This image is courtesy of an Abreview submitted by Zeng Li.
Western blot - PARP antibody (ab6079)
All lanes : Anti-PARP antibody (ab6079) at 1/400 dilution
Lane 1 : Lysate prepared from human HeLa cytosolic extracts
Lane 2 : Lysate prepared from human HeLa nuclear extracts
Lysates/proteins at 50 µg per lane.
Secondary
HRP-conjugated goat polyclonal to rabbit IgG at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 122 kDa
Observed band size : 29,112 kDa (why is the actual band size different from the predicted?)
Exposure time : 15 seconds
This image is a courtesy of Anonymous Abreview
References for Anti-PARP antibody (ab6079)
This product has been referenced in:
- Mehrotra PVet al. DNA Repair Factor APLF Is a Histone Chaperone. Mol Cell 41:46-55 (2011).Read more (PubMed: 21211722) »
- Tekcan Met al. Potential Role of Poly(ADP-Ribose) Polymerase Activation in the Pathogenesis of Experimental Left Varicocele. J Androl : (2011). WB, IHC-P; Rat.Read more (PubMed: 21436309) »
See all 7 publications for this product
Publishing research using ab6079? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"