Overview

  • Product nameAnti-PARP antibody
    See all PARP primary antibodies
  • Description
    Chicken polyclonal to PARP
  • SpecificityIn WB, ab75757 recognizes intact PARP (113kDa), the 89kDa apoptosis-induced cleavage product, and other lower molecular weight fragments of PARP.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length highly purified human PARP expressed in baculovirus.

  • Positive control
    • Jurkat cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab75757 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesELISA: Use at an assay dependent dilution.
    WB: Use at a concentration of 1 µg/ml (HRP/TMB colorimetric) or <1 µg/ml (ECL). Predicted molecular weight: 113 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionInvolved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150.
    • Sequence similaritiesContains 1 BRCT domain.
      Contains 1 PARP alpha-helical domain.
      Contains 1 PARP catalytic domain.
      Contains 2 PARP-type zinc fingers.
    • Post-translational
      modifications
      Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.
      Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
      S-nitrosylated, leading to inhibit transcription regulation activity.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase) antibody
      • ADP ribosyltransferase diphtheria toxin like 1 antibody
      • ADP ribosyltransferase NAD(+) antibody
      • ADPRT 1 antibody
      • ADPRT antibody
      • ADPRT1 antibody
      • ARTD1 antibody
      • msPARP antibody
      • NAD(+) ADP ribosyltransferase 1 antibody
      • NAD(+) ADP-ribosyltransferase 1 antibody
      • pADPRT 1 antibody
      • pADPRT1 antibody
      • PARP 1 antibody
      • PARP antibody
      • PARP-1 antibody
      • PARP1 antibody
      • PARP1_HUMAN antibody
      • Poly (ADP ribose) polymerase 1 antibody
      • poly (ADP ribose) polymerase family, member 1 antibody
      • Poly [ADP-ribose] polymerase 1 antibody
      • Poly(ADP ribose) polymerase antibody
      • poly(ADP ribose) synthetase antibody
      • poly(ADP ribosyl)transferase antibody
      • Poly[ADP ribose] synthetase 1 antibody
      • Poly[ADP-ribose] synthase 1 antibody
      • PPOL antibody
      see all

    Anti-PARP antibody images



    • Predicted band size : 113 kDa
      Lane 1 = Extract of HeLa cells treated with vehicle ? 20 ug. Lane 2 = Extract of HeLa cells treated with staurosporine ? 20 ug. Lane 3 = Extract of Jurkat cells treated with vehicle ? 20 ug. Lane 4 = Extract of Jurkat cells treated with staurosporine ? 20 ug. Lane 5 = Extract of C6 cells treated with vehicle ? 20 ug. Lane 6 = Extract of C6 cells treated with camptothecin ? 20 ug. Lane 7 = Extract of NIH 3T3 cells treated with vehicle ? 20 ug. Lane 8 = Extract of NIH 3T3 cells treated with staurosporine ? 20 ug. SDS PAGE performed under reducing conditions (100mM DTT ? Sample heated at 50?C). Primary : Lanes 1-8: Anti PARP antibody (ab75757) at 1:1000 dilution. Secondary : Lanes 1-8: Goat anti chicken IgY-HRP at 1:10000. Developed: ECL with 1 min exposure. Blocking: in 5% Milk + PBS for 3 hours at RT. Primary antibody: in 5% Milk + PBS overnight at 4 C. Secondary antibody: in 5% Milk + PBS for 2 hour at RT. Predicted band size : 113 kDa: 89 kDa and 24 kDa. Observed band size : 113 kDa: 89 kDa and 24 kDa.
    • All lanes : Anti-PARP antibody (ab75757) at 1 µg/ml

      Lane 1 : Jurkat cell lysate at 5 µg
      Lane 2 : Apoptotic Jurkat cell lysate, induced with camptothecin for 4 hr

      Secondary
      GAC-HRP at 1/2000 dilution

      Predicted band size : 113 kDa
      Additional bands at : 89 kDa (possible cleavage fragment).
      Note other smaller apoptosis-induced PARP fragments in Lane 2.

    References for Anti-PARP antibody (ab75757)

    ab75757 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us. We have several antibodies which can be used to detect PARP in Western blotting. From the study I believe you wish to detect both the cleaved (C-terminal fragment) and the full length PARP form in mouse samples? Thi...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"