The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Detects a band of approximately 115 kDa (predicted molecular weight: 113 kDa).
Application notesIs unsuitable for Flow Cyt,ICC or IHC.
FunctionInvolved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150.
Post-translational modificationsPhosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR. Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites. S-nitrosylated, leading to inhibit transcription regulation activity.
ADP ribosyltransferase diphtheria toxin like 1 antibody
ADP ribosyltransferase NAD(+) antibody
ADPRT 1 antibody
NAD(+) ADP ribosyltransferase 1 antibody
NAD(+) ADP-ribosyltransferase 1 antibody
pADPRT 1 antibody
PARP 1 antibody
Poly (ADP ribose) polymerase 1 antibody
poly (ADP ribose) polymerase family, member 1 antibody
Poly [ADP-ribose] polymerase 1 antibody
Poly(ADP ribose) polymerase antibody
poly(ADP ribose) synthetase antibody
poly(ADP ribosyl)transferase antibody
Poly[ADP ribose] synthetase 1 antibody
Poly[ADP-ribose] synthase 1 antibody
Anti-PARP antibody [Y17] images
Western blot - Anti-PARP antibody [Y17] (ab32378)
Predicted band size : 113 kDa
Lane 1: Wild type HAP1 whole cell lysate (20 µg) Lane 2: PARP1 knockout HAP1 whole cell lysate (20 µg) Lane 3: HeLa whole cell lysate (20 µg) Lane 4: MCF7 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab32378 observed at 125 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab32378 was shown to specifically react with PARP1 when PARP1 knockout samples were used. Wild-type and PARP1 knockout samples were subjected to SDS-PAGE. Ab32378 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) ab216773 and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Western blot - PARP antibody [Y17] (ab32378)
All lanes : Anti-PARP antibody [Y17] (ab32378) at 1/1000 dilution
Lane 1 : Jurkat cell lysate. Lane 2 : Jurkat cell lysate and Camptothecin cell lysate.