Overview

  • Product name
  • Description
    Rabbit polyclonal to PARP1
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    Synthetic peptide corresponding to Cow PARP1 (C terminal).

  • Positive control
    • WB: Raji cell lysate. IF/ICC: Hek293 cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab75607 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 5 - 10 µg/ml. Predicted molecular weight: 113 kDa.

Target

  • Function
    Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150.
  • Sequence similarities
    Contains 1 BRCT domain.
    Contains 1 PARP alpha-helical domain.
    Contains 1 PARP catalytic domain.
    Contains 2 PARP-type zinc fingers.
  • Post-translational
    modifications
    Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.
    Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
    S-nitrosylated, leading to inhibit transcription regulation activity.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase) antibody
    • ADP ribosyltransferase antibody
    • ADP ribosyltransferase diphtheria toxin like 1 antibody
    • ADP ribosyltransferase NAD(+) antibody
    • ADPRT 1 antibody
    • ADPRT antibody
    • ADPRT1 antibody
    • ARTD1 antibody
    • msPARP antibody
    • NAD(+) ADP ribosyltransferase 1 antibody
    • NAD(+) ADP-ribosyltransferase 1 antibody
    • pADPRT 1 antibody
    • pADPRT1 antibody
    • PARP 1 antibody
    • PARP antibody
    • PARP-1 antibody
    • PARP1 antibody
    • PARP1_HUMAN antibody
    • Poly (ADP ribose) polymerase 1 antibody
    • poly (ADP ribose) polymerase family, member 1 antibody
    • Poly [ADP-ribose] polymerase 1 antibody
    • Poly(ADP ribose) polymerase antibody
    • poly(ADP ribose) synthetase antibody
    • poly(ADP ribosyl)transferase antibody
    • Poly[ADP ribose] synthetase 1 antibody
    • Poly[ADP-ribose] synthase 1 antibody
    • PPOL antibody
    see all

Images



  • Predicted band size : 113 kDa

    Lane 1: HeLa cell extract treated with vehicle (20 µg).
    Lane 2: HeLa cells extract treated with staurosporine (20 µg).
    Lane 3: Jurkat cell extract treated with vehicle (20 µg).
    Lane 4: Jurkat cell extract treated with staurosporine (20 µg).

    SDS PAGE performed under reducing conditions (100mM DTT, sample heated at 50°C).

    Primary (Lanes 1-4): ab75607 at 2 µg/mL in 5% milk + PBS overnight at 4°C.
    Secondary (Lanes 1-4): HRP-conjugated goat anti-rabbit IgG (H&L) at 1/10000 in 5% milk + PBS for 2 hour at room temperature.

    Developed: ECL with 30 second exposure. Blocked in 5% milk + PBS for 3 hours at room temperature.

    Predicted band size: 113 kDa and 89 kDa.
    Observed band size: 113 kDa and 89 kDa.

  • ICC/IF image of ab75607 stained HEK293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab756007, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-PARP1 antibody (ab75607) at 10 µg/ml + Raji cell lysate

    Predicted band size : 113 kDa
    Observed band size : 113 kDa

References

This product has been referenced in:
  • Li Y  et al. Mechanism of the protective effects of the combined treatment with rhynchophylla total alkaloids and sinapine thiocyanate against a prothrombotic state caused by vascular endothelial cell inflammatory damage. Exp Ther Med 13:3081-3088 (2017). Read more (PubMed: 28587383) »
  • Qi P  et al. Rottlerin-induced autophagy leads to apoptosis in bladder cancer cells. Oncol Lett 12:4577-4583 (2016). Read more (PubMed: 28101215) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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