Overview

  • Product name
  • Description
    Chicken polyclonal to PARP1
  • Specificity
    In WB, ab75757 recognizes intact PARP1 (113kDa), the 89kDa apoptosis-induced cleavage product, and other lower molecular weight fragments of PARP1.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein corresponding to Human PARP1. Highly purified human PARP1 expressed in baculovirus.

  • Positive control
    • Jurkat cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab75757 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 113 kDa.

Use at a concentration of 1 µg/ml (HRP/TMB colorimetric) or <1 µg/ml (ECL). 

ELISA Use at an assay dependent concentration.

Target

  • Function
    Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150.
  • Sequence similarities
    Contains 1 BRCT domain.
    Contains 1 PARP alpha-helical domain.
    Contains 1 PARP catalytic domain.
    Contains 2 PARP-type zinc fingers.
  • Post-translational
    modifications
    Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.
    Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
    S-nitrosylated, leading to inhibit transcription regulation activity.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase) antibody
    • ADP ribosyltransferase antibody
    • ADP ribosyltransferase diphtheria toxin like 1 antibody
    • ADP ribosyltransferase NAD(+) antibody
    • ADPRT 1 antibody
    • ADPRT antibody
    • ADPRT1 antibody
    • ARTD1 antibody
    • msPARP antibody
    • NAD(+) ADP ribosyltransferase 1 antibody
    • NAD(+) ADP-ribosyltransferase 1 antibody
    • pADPRT 1 antibody
    • pADPRT1 antibody
    • PARP 1 antibody
    • PARP antibody
    • PARP-1 antibody
    • PARP1 antibody
    • PARP1_HUMAN antibody
    • Poly (ADP ribose) polymerase 1 antibody
    • poly (ADP ribose) polymerase family, member 1 antibody
    • Poly [ADP-ribose] polymerase 1 antibody
    • Poly(ADP ribose) polymerase antibody
    • poly(ADP ribose) synthetase antibody
    • poly(ADP ribosyl)transferase antibody
    • Poly[ADP ribose] synthetase 1 antibody
    • Poly[ADP-ribose] synthase 1 antibody
    • PPOL antibody
    see all

Images

  • All lanes : Anti-PARP1 antibody (ab75757) at 1/1000 dilution

    Lane 1 : Extract of HeLa cells treated with vehicle
    Lane 2 : Extract of HeLa cells treated with staurosporine
    Lane 3 : Extract of Jurkat cells treated with vehicle
    Lane 4 : Extract of Jurkat cells treated with staurosporine
    Lane 5 : Extract of C6 cells treated with vehicle
    Lane 6 : Extract of C6 cells treated with camptothecin
    Lane 7 : Extract of NIH 3T3 cells treated with vehicle
    Lane 8 : Extract of NIH 3T3 cells treated with staurosporine

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP-conjugated goat anti-chicken IgY at 1/1000 dilution
    Developed using the ECL technique

    Predicted band size : 113 kDa


    Exposure time : 1 minute

    SDS PAGE performed under reducing conditions (100mM DTT, Sample heated at 50°C).

    Blocking: in 5% Milk + PBS for 3 hours at RT.
    Primary antibody diluted in 5% Milk + PBS and incubated overnight at 4°C.
    Secondary antibody diluted in 5% Milk + PBS and incubated for 2 hour at RT.
    Predicted band size : 113 kDa: 89 kDa and 24 kDa.
    Observed band size : 113 kDa: 89 kDa and 24 kDa.

  • All lanes : Anti-PARP1 antibody (ab75757) at 1 µg/ml

    Lane 1 : Jurkat cell lysate at 5 µg
    Lane 2 : Apoptotic Jurkat cell lysate, induced with camptothecin for 4 hr

    Secondary
    GAC-HRP at 1/2000 dilution

    Predicted band size : 113 kDa
    Additional bands at : 89 kDa (possible cleavage fragment).

    Note other smaller apoptosis-induced PARP1 fragments in Lane 2.

References

ab75757 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for contacting us. We have several antibodies which can be used to detect PARP in Western blotting. From the study I believe you wish to detect both the cleaved (C-terminal fragment) and the full length PARP form in mouse samples? Thi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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