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Recombinant full length protein corresponding to Human PARP1. Highly purified human PARP1 expressed in baculovirus.
Our Abpromise guarantee covers the use of ab75757 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 113 kDa.
Use at a concentration of 1 µg/ml (HRP/TMB colorimetric) or <1 µg/ml (ECL).
|ELISA||Use at an assay dependent concentration.|
SDS PAGE performed under reducing conditions (100mM DTT, Sample heated at 50°C).
Blocking: in 5% Milk + PBS for 3 hours at RT.
Primary antibody diluted in 5% Milk + PBS and incubated overnight at 4°C.
Secondary antibody diluted in 5% Milk + PBS and incubated for 2 hour at RT.
Predicted band size : 113 kDa: 89 kDa and 24 kDa.
Observed band size : 113 kDa: 89 kDa and 24 kDa.
Note other smaller apoptosis-induced PARP1 fragments in Lane 2.
ab75757 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"