Overview

  • Product nameAnti-PARP11 antibody
    See all PARP11 primary antibodies
  • Description
    Rabbit polyclonal to PARP11
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide within residues: IKHGNTFQIH GVSLQQRHLF RTYKSMFLAR VLIGDYINGD SKYMRPPSKD , corresponding to internal sequence amino acids 253-302 of Human PARP11 (NP_065100)

  • Positive control
    • Human fetal muscle lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab84935 is purified by a peptide affinity chromatography method.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84935 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • RelevancePoly(ADP-ribosyl)ation is a DNA-damage-dependent post-translational modification of histones. Poly(ADP-ribose) polymerases (PARPs) are a family of 18 proteins, encoded by different genes and displaying a conserved catalytic domain.
  • Database links
  • Alternative names
    • 5330431N24Rik antibody
    • AI851877 antibody
    • C12orf6 antibody
    • DKFZp779H0122 antibody
    • HIN1L antibody
    • MIB006 antibody
    • OTTMUSP00000027219 antibody
    • Poly (ADP ribose) polymerase family member 11 antibody
    • RGD1561791 antibody
    see all

Anti-PARP11 antibody images

  • Anti-PARP11 antibody (ab84935) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal muscle lysate at 10 µg

    Secondary
    anti-Rabbit IgG HRP at 1/50000 dilution

    Predicted band size : 39 kDa
    Observed band size : 39 kDa
  • Anti-PARP11 antibody (ab84935) at 1 µg/ml + Recombinant human PARP11 protein (ab79742) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 12 minutes

References for Anti-PARP11 antibody (ab84935)

ab84935 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"