Overview

  • Product nameAnti-PARP14 antibody
    See all PARP14 primary antibodies
  • Description
    Rabbit polyclonal to PARP14
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human PARP14 (N terminal). UniProt ID: Q460N5 isoform 3.
    Database link: NM_017554

  • Positive control
    • WB: Human fetal brain lysate IHC-P: Human normal liver and fetal colon tissue. ICC/IF: A431 cells.

Properties

  • FormLyophilised:Add 200 ul sterile distilled water.
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab101010 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Predicted molecular weight: 171 kDa.
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionEnhances STAT6-dependent transcription (By similarity). Has ADP-ribosyltransferase activity.
  • Sequence similaritiesContains 3 Macro domains.
    Contains 1 PARP catalytic domain.
    Contains 1 WWE domain.
  • Cellular localizationNucleus. Cytoplasm. In steady state cells the protein is mostly nuclear. A minor proportion is detected in the cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADP-ribosyltransferase diphtheria toxin-like 8 antibody
    • ARTD8 antibody
    • B aggressive lymphoma protein 2 antibody
    • BAL2 antibody
    • Collaborator of STAT6 antibody
    • KIAA1268 antibody
    • PAR14_HUMAN antibody
    • PARP 14 antibody
    • PARP-14 antibody
    • PARP14 antibody
    • pART8 antibody
    • Poly (ADP ribose) polymerase family member 14 antibody
    • Poly [ADP-ribose] polymerase 14 antibody
    see all

Anti-PARP14 antibody images

  • ICC/IF image of ab101010 stained A431 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab101010 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human fetal colon tissue labelling PARP14 with ab101010 at a dilution of 1/100.

  • IHC image of PARP14 staining in Human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab101010, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-PARP14 antibody (ab101010) at 1/1000 dilution + Human fetal brain lysate

    Predicted band size : 171 kDa

References for Anti-PARP14 antibody (ab101010)

ab101010 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab101010.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"