Overview

  • Product name
  • Description
    Rabbit polyclonal to PARP16
  • Tested applications
    Suitable for: WB, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within internal amino acids 252-301 (PKYFVVTNNQ LLRVKYLLVY SQKPPKSRAS SQLSWFSSHW FTVMISLYLL) of Human PARP16 (NP_060321).

  • Positive control
    • 293T cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84641 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use at an assay dependent concentration. PubMed: 22701565
IP Use at an assay dependent concentration. PubMed: 22701565

Target

  • Sequence similarities
    Contains 1 PARP catalytic domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADP ribosyltransferase diphtheria toxin like 15 antibody
    • ADP-ribosyltransferase diphtheria toxin-like 15 antibody
    • ARTD 15 antibody
    • ARTD15 antibody
    • C15orf30 antibody
    • Chromosome 15 open reading frame 30 antibody
    • EC 2.4.2.30 antibody
    • FLJ20509 antibody
    • FLJ25281 antibody
    • mono [ADP-ribose] polymerase PARP16 antibody
    • mono ADP ribose polymerase PARP16 antibody
    • PAR16_HUMAN antibody
    • PARP 16 antibody
    • PARP-16 antibody
    • Parp16 antibody
    • pART 15 antibody
    • pART15 antibody
    • Poly (ADP ribose) polymerase family member 16 antibody
    • Poly [ADP ribose] polymerase 16 antibody
    • Poly [ADP-ribose] polymerase 16 antibody
    • poly ADP ribose polymerase 16 antibody
    see all

Images

  • Anti-PARP16 antibody (ab84641) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 36 kDa
    Observed band size : 36 kDa
  • Immunofluorescence analysis of HeLa cells, staining endogenous PARP16 using ab84641, at 1/50 dilution.
  • Immunofluorescence analysis of HeLa cells, staining endogenous PARP16 using ab84641, at 1/50 dilution.

References

This product has been referenced in:
  • Di Paola S  et al. PARP16/ARTD15 Is a Novel Endoplasmic-Reticulum-Associated Mono-ADP-Ribosyltransferase That Interacts with, and Modifies Karyopherin-ß1. PLoS One 7:e37352 (2012). WB, ICC/IF, IP ; Human . Read more (PubMed: 22701565) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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