Recombinant
RabMAb

Anti-PARP2 antibody [EPR12759(B)] (ab176330)

Overview

  • Product name
    Anti-PARP2 antibody [EPR12759(B)]
    See all PARP2 primary antibodies
  • Description
    Rabbit monoclonal [EPR12759(B)] to PARP2
  • Tested applications
    Suitable for: ICC/IF, WB, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PARP2 aa 500 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9UGN5

  • Positive control
    • Raji, HeLa, and fetal brain lysates
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab176330 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200.
WB 1/1000 - 1/5000. Predicted molecular weight: 66 kDa.
Flow Cyt 1/20.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function
      Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks.
    • Tissue specificity
      Widely expressed, mainly in actively dividing tissues. The highest levels are in the brain, heart, pancreas, skeletal muscle and testis; also detected in kidney, liver, lung, placenta, ovary and spleen; levels are low in leukocytes, colon, small intestine, prostate and thymus.
    • Sequence similarities
      Contains 1 PARP alpha-helical domain.
      Contains 1 PARP catalytic domain.
    • Post-translational
      modifications
      Poly-ADP-ribosylated by PARP1.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • ADP ribosyltransferase like 2 antibody
      • ADP-ribosyltransferase diphtheria toxin-like 2 antibody
      • ADPRT 2 antibody
      • ADPRT-2 antibody
      • ADPRT2 antibody
      • ADPRTL 2 antibody
      • ADPRTL 3 antibody
      • ADPRTL2 antibody
      • ADPRTL3 antibody
      • ARTD2 antibody
      • hPARP 2 antibody
      • hPARP-2 antibody
      • hPARP2 antibody
      • NAD(+) ADP ribosyltransferase 2 antibody
      • NAD(+) ADP-ribosyltransferase 2 antibody
      • pADPRT 2 antibody
      • pADPRT-2 antibody
      • pADPRT2 antibody
      • PARP 2 antibody
      • PARP-2 antibody
      • PARP2 antibody
      • PARP2_HUMAN antibody
      • Poly (ADP ribose) polymerase family member 2 antibody
      • Poly (ADP ribosyl) transferase like 2 antibody
      • poly (ADP-ribose) polymerase 2 antibody
      • Poly [ADP ribose] synthetase 2 antibody
      • Poly [ADP-ribose] polymerase 2 antibody
      • Poly(ADP ribose) synthetase antibody
      • Poly[ADP-ribose] synthase 2 antibody
      see all

    Images

    • ab176330 staining PARP2 in human HeLa cells treated with bleomycin by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100 in PBS. Samples were incubated with primary antibody (1/200 in PBS) for 16 hours at 22°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Counterstained with DAPI.

      See Abreview

    • All lanes : Anti-PARP2 antibody [EPR12759(B)] (ab176330) at 1/1000 dilution

      Lane 1 : Raji lysate
      Lane 2 : HeLa lysate
      Lane 3 : Fetal brain lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 66 kDa
    • ab176330 staining PARP2 in the human cell line HeLa (human cervix adenocarcinoma) by flow cytometry. Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/20. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

      Isoytype control: Rabbit monoclonal IgG (Black)

      Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

    References

    This product has been referenced in:
    • Ye Y  et al. Up-regulation of REG3A in colorectal cancer cells confers proliferation and correlates with colorectal cancer risk. Oncotarget 7:3921-33 (2016). WB . Read more (PubMed: 26646797) »
    • Faraoni I  et al. BRCA1, PARP1 and ?H2AX in acute myeloid leukemia: Role as biomarkers of response to the PARP inhibitor olaparib. Biochim Biophys Acta 1852:462-72 (2015). Read more (PubMed: 25483710) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa (see notes for treatment))
    Specification
    HeLa (see notes for treatment)
    Permeabilization
    Yes - 0.5% Triton X100 in PBS
    Fixative
    Paraformaldehyde
    Username

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Jan 23 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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