Overview

  • Product nameAnti-PARP4 antibody
    See all PARP4 primary antibodies
  • Description
    Rabbit polyclonal to PARP4
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 1674-1724 (

    EWVRRTEGQY PSICPRLELG NDWDSATKQL LGLQPISTVS PLHRVLHYSQG

    ) of Human PARP4 (NP_006428.2)

  • Positive control
    • Hela cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab86066 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 193 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • RelevanceComponent of the vault ribonucleoprotein particle which is at least composed of MVP, PARP4 and one or more vault RNAs (vRNAs). Binds to MVP. Associates with TEP1. Widely expressed; the highest levels are in the kidney; also detected in heart, placenta, lung, liver, skeletal muscle, spleen, leukocytes and pancreas.
  • Cellular localizationCytoplasmic, but is also found in the nucleus, associated with mitotic spindles.
  • Database links
  • Alternative names
    • 193kDa vault protein antibody
    • ADP ribosyltransferase (NAD+ poly (ADP ribose) polymerase) like 1 antibody
    • ADP ribosyltransferase like 1 antibody
    • ADP-ribosyltransferase diphtheria toxin-like 4 antibody
    • ADPRT L1 antibody
    • ADPRTL 1 antibody
    • ADPRTL1 antibody
    • ARTD4 antibody
    • H5 proline rich antibody
    • I alpha I related antibody
    • KIAA0177 antibody
    • Minor vault p193 protein antibody
    • OTTHUMP00000042288 antibody
    • p193 antibody
    • PARP 4 antibody
    • PARP related / IalphaI related H5 / proline rich antibody
    • PARP related antibody
    • PARPL antibody
    • PH 5P antibody
    • PH5P antibody
    • Poly (ADP ribose) polymerase 4 antibody
    • Poly (ADP ribose) polymerase family member 4 antibody
    • Poly (ADP ribose) synthetase antibody
    • Poly (ADP ribosyl) transferase like 1 antibody
    • Poly ADP ribose polymerase 4 antibody
    • Poly ADP ribose polymerase family member 4 antibody
    • Vault 3 antibody
    • Vault antibody
    • Vault poly (ADP ribose) polymerase antibody
    • Vault poly ADP ribose polymerase antibody
    • Vault protein 193 kDa antibody
    • Vault3 antibody
    • von Willebrand factor A domain containing 5C antibody
    • VPARP antibody
    • VWA5C antibody
    see all

Anti-PARP4 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling PARP4 with ab86066 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-PARP4 antibody (ab86066) at 0.04 µg/ml

    Lane 1 : Hela whole cell lysate at 50 µg
    Lane 2 : Hela whole cell lysate at 15 µg
    Lane 3 : Hela whole cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 193 kDa


    Exposure time : 3 minutes
  • 1 mg whole cell lysate from HeLa cells was immunoprecipitated using 3 µg of ab86066. 20% of IP was loaded in each lane and probed with ab86066 at 0.4 µg/ml (lane 1) or with a control IgG (lane 2).
    Detection: chemiluminescence with an exposure time of 30 seconds.

References for Anti-PARP4 antibody (ab86066)

ab86066 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"