Overview

  • Product nameAnti-PARP4 antibody [P193-10]
    See all PARP4 primary antibodies
  • Description
    Mouse monoclonal [P193-10] to PARP4
  • Specificityab24110 reacts with an internal epitope (amino acids 506-510, VALGK) of the PARP4, which is overexpressed in various human non-P-glycoprotein MDR tumor cell lines.
  • Tested applicationsSuitable for: WB, Flow Cyt, IHC-P, IHC-Fr, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to amino acids 408-611 of Human PARP4.

  • Positive control
    • IHC-P: FFPE human kidney tissue sections.

Properties

Applications

Our Abpromise guarantee covers the use of ab24110 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Flow Cyt
IHC-P
IHC-Fr
IHC-FoFr
  • Application notesFlow Cyt: Use at an assay dependent dilution.
    IHC-F: 1/10 - 1/20.
    IHC-Fr: 1/10 - 1/20.
    IHC-P: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. preferably with ECL
    Predicted molecular weight: 193 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceComponent of the vault ribonucleoprotein particle which is at least composed of MVP, PARP4 and one or more vault RNAs (vRNAs). Binds to MVP. Associates with TEP1. Widely expressed; the highest levels are in the kidney; also detected in heart, placenta, lung, liver, skeletal muscle, spleen, leukocytes and pancreas.
    • Cellular localizationCytoplasmic, but is also found in the nucleus, associated with mitotic spindles.
    • Database links
    • Alternative names
      • 193kDa vault protein antibody
      • ADP ribosyltransferase (NAD+ poly (ADP ribose) polymerase) like 1 antibody
      • ADP ribosyltransferase like 1 antibody
      • ADP-ribosyltransferase diphtheria toxin-like 4 antibody
      • ADPRT L1 antibody
      • ADPRTL 1 antibody
      • ADPRTL1 antibody
      • ARTD4 antibody
      • H5 proline rich antibody
      • I alpha I related antibody
      • KIAA0177 antibody
      • Minor vault p193 protein antibody
      • OTTHUMP00000042288 antibody
      • p193 antibody
      • PARP 4 antibody
      • PARP related / IalphaI related H5 / proline rich antibody
      • PARP related antibody
      • PARPL antibody
      • PH 5P antibody
      • PH5P antibody
      • Poly (ADP ribose) polymerase 4 antibody
      • Poly (ADP ribose) polymerase family member 4 antibody
      • Poly (ADP ribose) synthetase antibody
      • Poly (ADP ribosyl) transferase like 1 antibody
      • Poly ADP ribose polymerase 4 antibody
      • Poly ADP ribose polymerase family member 4 antibody
      • Vault 3 antibody
      • Vault antibody
      • Vault poly (ADP ribose) polymerase antibody
      • Vault poly ADP ribose polymerase antibody
      • Vault protein 193 kDa antibody
      • Vault3 antibody
      • von Willebrand factor A domain containing 5C antibody
      • VPARP antibody
      • VWA5C antibody
      see all

    Anti-PARP4 antibody [P193-10] images

    • IHC image of PARP4 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab24110, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    References for Anti-PARP4 antibody [P193-10] (ab24110)

    This product has been referenced in:
    • Li B  et al. Conditional Akt activation promotes androgen-independent progression of prostate cancer. Carcinogenesis 28:572-83 (2007). Read more (PubMed: 17032658) »

    See 1 Publication for this product

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