Overview

  • Product name
  • Description
    Rabbit polyclonal to PARP7
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 36-85 (LKTCFKKKDQ KRLGTGTLRS LRPILNTLLE SGSLDGVFRS RNQSTDENSL) of Human PARP7 (NP_056323).

  • Positive control
    • Human fetal heart lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84664 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 23275542
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 76 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Poly [ADP-ribose] polymerase using NAD(+) as a substrate to transfer ADP-ribose onto glutamic acid residues of a protein acceptor; repeated rounds of ADP-ribosylation leads to the formation of poly(ADPribose) chains on the protein, thereby altering the function of the target protein. May play a role in the adaptative response to chemical exposure (TCDD) and thereby mediates certain effects of the chemicals.
  • Sequence similarities
    Contains 1 C3H1-type zinc finger.
    Contains 1 PARP catalytic domain.
    Contains 1 WWE domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADP-ribosyltransferase diphtheria toxin-like 14 antibody
    • ARTD14 antibody
    • AW558171 antibody
    • DDF1 antibody
    • DKFZp434J214 antibody
    • DKFZp686N0351 antibody
    • DKFZp686P1838 antibody
    • FLJ40466 antibody
    • PARP 1 antibody
    • PARP 7 antibody
    • PARP-7 antibody
    • PARPT_HUMAN antibody
    • pART14 antibody
    • Poly [ADP ribose] polymerase 7 antibody
    • Poly [ADP-ribose] polymerase 7 antibody
    • TCDD inducible poly(ADP ribose) polymerase antibody
    • TCDD-inducible poly [ADP-ribose] polymerase antibody
    • TIPARP antibody
    see all

Images

  • Anti-PARP7 antibody (ab84664) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal heart lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 76 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Macpherson L  et al. 2,3,7,8-Tetrachlorodibenzo-p-dioxin poly(ADP-ribose) polymerase (TiPARP, ARTD14) is a mono-ADP-ribosyltransferase and repressor of aryl hydrocarbon receptor transactivation. Nucleic Acids Res 41:1604-21 (2013). ICC/IF . Read more (PubMed: 23275542) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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