1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Involved in inducing the expression of IFN-gamma-responsive genes.
Expressed in lymphocyte-rich tissues, spleen, lymph nodes, peripheral blood lymphocytes and colonic mucosa. Also expressed in nonhematopoietic tissues such as heart and skeletal muscle. Isoform 2 is the predominant form. Most abundantly expressed in lymphomas with a brisk host inflammatory response. In diffuse large B-cell lymphomas tumors, expressed specifically by malignant B-cells.
Involvement in disease
Note=Overexpressed at significantly higher levels in fatal high-risk diffuse large B-cell lymphomas (DLB-CL) compared to cured low-risk tumors. Overexpression in B-cell lymphoma transfectants may promote malignant B-cell migration. May therefore be involved in promoting B-cell migration and dissemination of high-risk DLB-CL tumors.
Immunohistochemical analysis of formalin fixed and paraffin embedded Human lymphoma labeling PARP9 with ab170481 at 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining
Western blot - Anti-PARP9 antibody - C-terminal (ab170481)
Anti-PARP9 antibody - C-terminal (ab170481) at 1/100 dilution + mouse lung tissue lysate at 35 µg