The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100. Predicted molecular weight: 97 kDa.
1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Involved in inducing the expression of IFN-gamma-responsive genes.
Expressed in lymphocyte-rich tissues, spleen, lymph nodes, peripheral blood lymphocytes and colonic mucosa. Also expressed in nonhematopoietic tissues such as heart and skeletal muscle. Isoform 2 is the predominant form. Most abundantly expressed in lymphomas with a brisk host inflammatory response. In diffuse large B-cell lymphomas tumors, expressed specifically by malignant B-cells.
Involvement in disease
Note=Overexpressed at significantly higher levels in fatal high-risk diffuse large B-cell lymphomas (DLB-CL) compared to cured low-risk tumors. Overexpression in B-cell lymphoma transfectants may promote malignant B-cell migration. May therefore be involved in promoting B-cell migration and dissemination of high-risk DLB-CL tumors.
Immunohistochemical analysis of formalin fixed, paraffin embedded Human rectum tissue labeling PARP9 with ab170609 at 1/10 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot - Anti-PARP9 antibody - N-terminal (ab170609)
Anti-PARP9 antibody - N-terminal (ab170609) at 1/50 dilution + Mouse liver tissue lysate at 35 µg