Overview

  • Product name
  • Description
    Rabbit polyclonal to Parvalbumin
  • Tested applications
    Suitable for: IHC-FoFr, IHC-Fr, WB, ELISA, IHC-P, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Human, Sea urchin
    Predicted to work with: Gerbil
  • Immunogen

    Full length native protein (purified) corresponding to Rat Parvalbumin. Purified parvalbumin from rat skeletal muscle.

  • Positive control
    • Rat cerebellum.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 3% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab11427 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration. PubMed: 20631843
IHC-Fr 1/2000.
WB Use a concentration of 0.1 µg/ml. Detects a band of approximately 12 kDa. Parvalbumin protein is relatively small and, therefore, it is recommended that electrophoresis be performed using tricine-SDS-PAGE gels and transferred to a nylon membrane.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 1 µg/ml.
IP Use a concentration of 5 µg/ml.
ICC/IF 1/100 - 1/200.

Target

Anti-Parvalbumin antibody images

  • Anti-Parvalbumin antibody (ab11427) + rat cerebellum extract
  • Immunocytochemistry/Immunofluorescence analysis of U251 cells labeling Parvalbumin (green) with ab11427 at 1/200. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.

  • Immunohistochemistry was performed on normal biopsies of deparaffinized Human tonsil tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a rabbit polyclonal antibody recognizing Parvalbumin ab11427 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labeling Parvalbumin (green) with ab11427 at 1/100. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.

  • Immunocytochemistry/Immunofluorescence analysis of C6 cells labeling Parvalbumin (green) with ab11427 at 1/100. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.

  • Immunocytochemcial immunofluorescence analysis of 4% PFA & 0.2% Picric acid fixed rat cordical cells in culture, labelling parvalbumin with ab11427 at a dilution of 1/500 incubated for 12 hours at 4°C in 10mM PBS & 0.03% Triton X diluent blend. The secondary was a Donkey anti-Rabbit polyclonal Alexa Fluor® 488 conjugate at 1/200.

    See Abreview

  • ab11427 staining mouse brain cells by ICC/IF.  Cells were PFA fixed and permeabilized with Triton, prior to blocking with 10% serum for 1 hour at RT.  The primary antibody was used undiluted and incubated with the sample for 18 hours at 4°C.  An Alexa Fluor® 488 conjugated goat anti-rabbit antibody was used as the secondary.

    See Abreview

  • ab11427 staining Parvalbumin in human brain tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed in formaldehyde and a heat mediated antigen retrieval step was performed using EDTA pH 8.0 for 20 minutes at 100°C. Samples were then incubated with ab11427 at a 1/1000 dilution for 20 minutes at 25°C. The secondary used was an undiluted HRP conjugated goat anti-mouse/ rabbit IgG.

    See Abreview

  • Immunohistochemistry was performed on normal biopsies of deparaffinized Human cerebellum tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a rabbit polyclonal antibody recognizing Parvalbumin ab114227 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Immunohistochemistry was performed on normal biopsies of deparaffinized Human cerebellum tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a rabbit polyclonal antibody recognizing Parvalbumin ab114227 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Immunohistochemistry was performed on normal biopsies of deparaffinized Human skeletal muscle tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyclonal antibody recognizing Parvalbumin ab11427 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

References for Anti-Parvalbumin antibody (ab11427)

This product has been referenced in:
  • Subashini C  et al. Wnt5a is a crucial regulator of neurogenesis during cerebellum development. Sci Rep 7:42523 (2017). IHC-P ; Mouse . Read more (PubMed: 28205531) »
  • Murphy S  et al. Proteomic profiling of mdx-4cv serum reveals highly elevated levels of the inflammation-induced plasma marker haptoglobin in muscular dystrophy. Int J Mol Med 39:1357-1370 (2017). Read more (PubMed: 28440464) »

See all 37 Publications for this product

Product Wall

Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Username

Abcam user community

Verified customer

Submitted Jan 12 2016

Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Username

Abcam user community

Verified customer

Submitted Jan 14 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (neurons of human cerebral organoid)
Permeabilization
Yes - Triton X-100
Specification
neurons of human cerebral organoid
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 37°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jul 12 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Whole brain sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
Yes - 0.05% Tween-20
Specification
Whole brain sections
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jun 02 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Whole brain tissue lysate)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (8-12% Bis-Tris)
Loading amount
20 µg
Specification
Whole brain tissue lysate
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

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Verified customer

Submitted Jun 02 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Brain)
Permeabilization
Yes - 0.2% triton-X100 in PBS
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 37°C
Fixative
Paraformaldehyde
Username

Miss. Berta Anuncibay

Verified customer

Submitted May 05 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (cerebral cortex)
Permeabilization
Yes - 0.1% TritonX-100 / PBS
Specification
cerebral cortex
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Weihua Wang​

Verified customer

Submitted Apr 07 2017

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Pig Tissue sections (Brain)
Antigen retrieval step
None
Permeabilization
Yes - Triton
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative
Formalin
Username

Abcam user community

Verified customer

Submitted Sep 28 2016

Application
IHC - Wholemount
Sample
Mouse Tissue (Brain, cerebellum)
Specification
Brain, cerebellum
Username

Abcam user community

Verified customer

Submitted May 20 2016

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain)
Permeabilization
Yes - 0.1% Triton X-100
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Dec 17 2015

1-10 of 29 Abreviews or Q&A

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