Anti-Parvalbumin antibody (ab32895)

Overview

  • Product nameAnti-Parvalbumin antibody
    See all Parvalbumin primary antibodies
  • Description
    Goat polyclonal to Parvalbumin
  • Tested applicationsSuitable for: WB, ELISA, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Xenopus laevis, Macaque Monkey
  • Immunogen

    Synthetic peptide:

    GVDEFSTLVAES

    , corresponding to C terminal amino acids 98-109 of Human Parvalbumin

  • Positive control
    • Human Brain lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab32895 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).
ELISA 1/32000.
IHC-Fr Use at an assay dependent concentration.

Target

Anti-Parvalbumin antibody images

  • Anti-Parvalbumin antibody (ab32895) at 0.1 µg/ml + Human Brain lysate at 35 µg

    Predicted band size : 12 kDa
    Observed band size : 12 kDa
  • Anti-Parvalbumin antibody (ab32895) at 0.2 µg/ml + whole murine brain tissue lysate at 20 µg

    Secondary
    Rabbit Anti-Goat IgG H&L (HRP) (ab6741) at 1/8000 dilution
    Developed using the ECL technique

    Predicted band size : 12 kDa
    Observed band size : 14 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes

    Image courtesy of an anonymous Abreview.

    See Abreview

References for Anti-Parvalbumin antibody (ab32895)

ab32895 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 27°C
Sample Mouse Tissue sections (frozen brain section)
Specification frozen brain section
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 07 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brain (hippocampus))
Specification Brain (hippocampus)
Fixative Paraformaldehyde
Permeabilization No
Username

Abcam user community

Verified customer

Submitted Dec 19 2012

Merci de nous avoir contactés. L'anti-Parvalbumine d'Abcam, référence ab32895, est un polyclonal de chèvre alors que l'anticorps d'Alpha Diagnostic est un monoclonal de souris. J'espère que ces informations vous seront utiles. N'hésitez pas à nou...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - other (brain)
Loading amount 20 µg
Specification brain
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 15 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"