Overview

  • Product name
  • Description
    Goat polyclonal to Parvalbumin
  • Tested applications
    Suitable for: WB, ELISA, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Xenopus laevis, Macaque monkey
  • Immunogen

    Synthetic peptide:

    GVDEFSTLVAES

    , corresponding to C terminal amino acids 98-109 of Human Parvalbumin

  • Positive control
    • Human Brain lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab32895 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).
ELISA 1/32000.
IHC-Fr Use at an assay dependent concentration.

Target

Images

  • Anti-Parvalbumin antibody (ab32895) at 0.1 µg/ml + Human Brain lysate at 35 µg

    Predicted band size : 12 kDa
    Observed band size : 12 kDa
  • Anti-Parvalbumin antibody (ab32895) at 0.2 µg/ml + whole murine brain tissue lysate at 20 µg

    Secondary
    Rabbit Anti-Goat IgG H&L (HRP) (ab6741) at 1/8000 dilution
    Developed using the ECL technique

    Predicted band size : 12 kDa
    Observed band size : 14 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes

    Image courtesy of an anonymous Abreview.

    See Abreview

References

This product has been referenced in:
  • Eshima H  et al. Long-term, but not short-term high-fat diet induces fiber composition changes and impaired contractile force in mouse fast-twitch skeletal muscle. Physiol Rep 5:N/A (2017). Read more (PubMed: 28408640) »
  • Tomer R  et al. Advanced CLARITY for rapid and high-resolution imaging of intact tissues. Nat Protoc 9:1682-97 (2014). Read more (PubMed: 24945384) »

See all 2 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 27°C
Sample
Mouse Tissue sections (frozen brain section)
Specification
frozen brain section
Permeabilization
No
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 07 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain (hippocampus))
Specification
Brain (hippocampus)
Fixative
Paraformaldehyde
Permeabilization
No
Username

Abcam user community

Verified customer

Submitted Dec 19 2012

Merci de nous avoir contactés. L'anti-Parvalbumine d'Abcam, référence ab32895, est un polyclonal de chèvre alors que l'anticorps d'Alpha Diagnostic est un monoclonal de souris. J'espère que ces informations vous seront utiles. N'hésitez pas à nou...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - other (brain)
Loading amount
20 µg
Specification
brain
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 15 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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