SpecificityParvalbumin. Immunoreactivity is seen primarily in Purkinje cells, basket and stellate cells. Immunostaining is completely eliminated when the antibody is incubated with the immunogen peptide prior to staining.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIHC-P: 1/100.
10% neutral Buffered Formalin fixed human cerebellum tissue. Suggested pretreatment: heat mediated antigen retrieval using Citrate buffer, pH 6.0. Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionIn muscle, parvalbumin is thought to be involved in relaxation after contraction. It binds two calcium ions.
Sequence similaritiesBelongs to the parvalbumin family. Contains 2 EF-hand domains.