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Rabbit polyclonal to PASK
Predicted to work with:
Synthetic peptide, corresponding to a region within amino acids 425-475 of Human PASK (NP_055963.2).
HeLa, 293T and Jurkat whole cell lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate Note: pH 7 to 8
Concentration information loading...
Immunogen affinity purified
ab114988 was affinity purified using an epitope specific to PASK immobilized on solid support.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 143 kDa.
Use at 2-10 µg/mg of lysate.
Ubiquitously expressed, with slightly higher expression in brain, prostate and testis. Reduced expression was found in placenta.
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
Contains 2 PAS (PER-ARNT-SIM) domains. Contains 1 protein kinase domain.
Autophosphorylated on Thr-1161 and Thr-1165.
Information by UniProt
Western blot - Anti-PASK antibody (ab114988)
All lanes : Anti-PASK antibody (ab114988) at 0.04 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : 293T whole cell lysate at 50 µg Lane 4 : Jurkat whole cell lysate at 50 µg Developed using the ECL technique Predicted band size : 143 kDa Exposure time : 3 minutes
Immunoprecipitation - Anti-PASK antibody (ab114988)
Detection of PASK by Western Blot of Immunprecipitate.
ab114988 at 0.4µg/ml staining PASK in HeLa whole cell lysate immunoprecipitated using ab114988 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 3 mminutes.
has not yet been referenced specifically in any publications.
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