Overview

  • Product name
  • Description
    Rabbit polyclonal to PASK
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 425-475 of Human PASK (NP_055963.2).

  • Positive control
    • HeLa, 293T and Jurkat whole cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7 to 8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab114988 was affinity purified using an epitope specific to PASK immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab114988 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 143 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Tissue specificity
    Ubiquitously expressed, with slightly higher expression in brain, prostate and testis. Reduced expression was found in placenta.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
    Contains 2 PAS (PER-ARNT-SIM) domains.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated on Thr-1161 and Thr-1165.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZP434O051 antibody
    • DKFZp686P2031 antibody
    • hPASK antibody
    • KIAA0135 antibody
    • MGC93882 antibody
    • mKIAA0135 antibody
    • PAS domain containing serine/threonine kinase antibody
    • PAS domain containing serine/threonine protein kinase antibody
    • PAS domain-containing serine/threonine-protein kinase antibody
    • PAS kinase antibody
    • PAS serine/threonine kinase antibody
    • PAS-kinase antibody
    • Pask antibody
    • PASK_HUMAN antibody
    • PASKIN antibody
    • STK 37 antibody
    • STK37 antibody
    see all

Images

  • All lanes : Anti-PASK antibody (ab114988) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 143 kDa


    Exposure time : 3 minutes
  • Detection of PASK by Western Blot of Immunprecipitate.
    ab114988 at 0.4µg/ml staining PASK in HeLa whole cell lysate immunoprecipitated using ab114988 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 3 mminutes.

References

ab114988 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab114988.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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